1909.] Destiny of Cholesterol in the Animal Organism. 511 





First day. 



Third day. 



Sixth day. 



Ninth day. 





lbs. ozs. 



lbs. ozs. 



lbs. ozs. 



lbs. ozs. 



Cat I 



7 14 



7 14 



8 



8 2 



„ II 



7 6 



7 6 



7 4 



7 2 



„ HI 



6 14 



6 10 



6 4 



6 2 



„ IV 



5 14 



5 12 



5 6 



5 4 



crystalline matter were obtained, which melted at 135° — 137° C. This 

 consisted mainly of cholesterol, for a portion, after recrystallisation again from 

 alcohol, melted at 142° C, and another portion, on treatment in ether acetic 

 acid solution with bromine, according to Windaus' method, gave cholesterol 

 dibromide, melting at 120° — 122° C, in fair yield. The mother liquors, after 

 recrystallisation from alcohol, yielded a small amount of matter, which 

 under the microscope had the appearance of a mixture of cholesterol and 

 phytosterol. The residues, after separating the above-mentioned 14495 

 grammes of cholesterol, were benzoylated in pyridine solution and - 5049 

 gramme of fairly clean benzoate was obtained. This melted, after recrystalli- 

 sation from ethyl acetate at 146° — 147° C, to a turbid liquid, which cleared 

 at 170° and on cooling gave a brilliant display of colours: Reckoning the 

 whole of the crystalline matter as cholesterol, 1*5472 grammes were obtained, 

 corresponding to a daily output of about O04 gramme ; or, if we subtract the 

 quantity of cholesterol contained in the fat given with the wheat germ, which 

 amounted in all to - 36 gramme, the daily output, independent of food, was 

 - 033 gramme. No trace of coprosterol was discovered. 



VIII. A cat, weighing l - 7 kilogrammes, was fed on a diet prepared similarly 

 to the last, but without any fat, for 17 days. It consumed altogether 

 630 grammes of extracted germ of wheat, and produced 93 grammes of dried 

 faeces. The weight of the unsaponifiable matter was - 6930 gramme and 

 fairly crystalline. From this (V5495 gramme of cholesterol was obtained, 

 corresponding to an output per day of about 0"03 gramme. 



IX. A cat was fed as in Experiment VIII for eight days, but during the 

 first five days it received, mixed with its food, small quantities of pure 

 phytosterol. It consumed altogether 250 grammes of the extracted germ 

 and 1-41 grammes of phytosterol. The weight of dry fasces was 68 grammes 

 and this yielded T7415 grammes of unsaponifiable matter as a greasy 

 crystalline solid. On crystallisation from alcohol, T3545 grammes of white 

 crystalline matter, which appeared to consist of almost pure phytosterol. An 

 attempt was made to separate any cholesterol from this by conversion into 

 the di bromides by Windaus' method, but without success. A small quantity 

 of dibromide separated out on standing after the addition of the acetic acid 



