528 Mr. W. J. Young. Hexosejjhosphate formed by [July 30 r 



2. Chloroform is not decomposed in the blood with the formation of carbon 

 monoxide. 



3. [October 12, 1909. — " The iodine liberated in the experiments of Desgrez 

 and Nicloux on anaesthetised animals was due to some extent to the direct- 

 decomposition of the iodine pentoxide by the chloroform vapour contained in 

 their blood gases, but mainly to the carbon monoxide produced by the action 

 of this chloroform on the potash over which they passed the blood gases in- 

 order to free them from carbon dioxide. This explanation is quite in accord- 

 ance with their observations that the amount of iodine liberated increases 

 with the duration of the anaesthesia."] 



We express our thanks to the Government Grant Committee of the Royal 

 Society for the funds which they have placed at our disposal for our work. 



The HexQsei^hosjpJiate formed by Yeast-juice from Hexose and 



Pliosphate. 



By W. J. Young (Biochemical Laboratory of the Lister Institute of 

 Preventive Medicine). 



(Communicated by A. Harden, F.R.S. Received July 30, 1909.) 



It has been shown by Harden and Young* that — (1) the rate of fermenta- 

 tion of glucose by yeast-juice is greatly increased by the addition of a soluble 

 phosphate ; (2) this rate soon diminishes until a constant rate is attained, 

 which is only slightly greater than that of the original yeast-juice and 

 glucose ; (3) during this period of increased fermentative activity, the 

 phosphate undergoes some alteration, and at the end of the period is no 

 longer present in a form precipitable by magnesium citrate mixture. The 

 authors suggested in the first paper that a combination of the phosphate with 

 the sugar, to form a phosphoric acid ester, had taken place, and more recently!" 

 embodied this suggestion in the form of an equation — 



2C 6 H 12 6 + 2R' 2 HP0 4 = 2C0 2 + 2C 2 H 6 + C 6 H 10 O4(PO4R' 2 ) 2 + 2H 2 0. 



It has also been shown that the same phenomena occur when fructose or- 

 mannose is used in place of glucose.} 



* 'Chem. Soc. Proc.,' 1905, vol. 21, p. 189 ; ' Koy. Soc. Proc.,' B, 1906, vol. 77, p. 405- 

 t 'Boy. Soc. Proc.,' B, 1908, vol. 80, p. 299. 



\ ' Chem. Soc. Proc.,' 1908, vol. 24, p. 115 ; ' Roy. Soc. Proc.,' B, vol. 81, 1909. 



