536 Mr. W. J. Young. Hexose phosphate Jormed by [July 30, 



with glucosazone melted at 206° to 207°. The analysis also corresponded to 

 that of glucosazone — 



01222 gramme gave 16 - 2 c.c. nitrogen at 16 0- 4 and 774 - 3 mm. 

 N = 1576 per cent. 



Glucosazone, CigE^C^N,!, requires N = 15'64 per cent. 



The fructose and mannose acids, when treated as above, yielded syrups 

 having the same properties as that from the glucose acid ; in both cases 

 iducosazone was obtained from them. 



All these syrups were found to induce rapid fermentation in a mixture of 

 yeast-juice and glucose with such an excess of phosphate that fermentation 

 was only proceeding at a very slow rate, and this induction has been shown 

 to be brought about by the addition of fructose, but not of glucose or 

 mannose.* 



Finally, the ratio of the rotation to the reducing power was obtained and 



was found to be approximately the same as that found for pure fructose. 



The reducing power was determined by means of Pavy's ammoniacal copper 



solution and, as this was standardised, each time it was used, with a solution 



of pure glucose it has been found convenient to express the reduction per 



100 c.c. of solution by the number of grammes of glucose which would reduce 



the same amount of copper, and to compare the rotation of the solution in 



, , . , , . , _ __ _ _ . . . Rotation 



a 4-dm. tube with this number. In lable Vlll. this ratio, — r- — .is 



Reduction 



given for the hexoses obtained by means of milk of lime from the products of 

 hydrolysis of the acids derived from all three sugars, and it will be seen that 

 it agrees in every case fairly well with that found for pure fructose. 



Table VIII. 



Sugar from — 



— Rotation in 

 4 dm. tube. 



Reduction as glucose 

 per 100 c.c. 



Ratio. 





o 



1 -806 



0-512 



3-52 





-962 



0-284 



3-38 



„ . (2) 



0-516 



0-151 



3 -41 





1-376 



0-441 



3-12 





0-607 



j -161 



3-77 



(2) 



-829 



0-219 



3 79 



The solutions, after removal of the phosphate and hexosephosphate and 

 before the treatment with milk of linie, always possessed a greater reducing 



* Harden and Young, ' Chem. Soc. Proc., J 1908, vol. 24, p. 115 ; ' Koy. Soc. Proa, 

 B, 1909. 



