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1909.] Yeast-juice from Hexose and Phosphate. 539 



Barium hexosephosphate, C«Hio04(P0 4 Ba) 2 , was obtained by adding a 

 solution of barium chloride to a solution of potassium or sodium hexose- 

 phosphate, and warming the mixture on the water bath. A granular white 

 precipitate was thus formed, which was found to be more soluble in cold 

 than in hot water. This was purified by dissolving in cold water and 

 reprecipitating by warming the solution. It was then filtered off, washed 

 with warm water, and dried over sulphuric acid in a vacuum. At 17°'5 

 100 c.c. of solution were found to contain 0'665, - 692, and 0*656 gramme of 

 the barium salts derived from glucose, fructose, and mannose respectively 

 The following analyses were obtained for this salt : — 



Table XI. 



Origin. 



Weight 

 taken. 



BaS0 4 . 



MgjP,0 7 . 



Percei 

 Ba. 



itages. 

 P. 



» (2) 



Fructose (1) 



•U> (2) . - 



-2578 

 f 0-5298 

 \ 0-4317 

 -4009 

 -5221 

 -4186 



-1994 

 -4002 



-3081 

 -3933 

 -3179 



-0960 



-1538 

 -1485 

 -1902 

 -1538 



45 -50 



44 "46 



45 -23 

 44-34 

 44-70 



10 -36 



9-92 

 10 -32 

 10 -14 

 10 -22 





44 -92 



10 -16 



Calcium Hexosephosphate. — A calcium salt of the acid derived from glucose 

 was obtained by adding calcium chloride to a solution of the sodium salt, 

 and completing the precipitation by adding alcohol. When dried over 

 sulphuric acid in a vacuum it gave the following analysis : — 



0-3488 gramme gave 0-1958 gramme C0 2 , 0-0917 gramme H 2 0, 

 0-0890 gramme CaO, and 0-1778 gramme Mg 2 P 2 7 . 



C = 16-81, H = 2-92, Ca = 18-24, P = 14-20 per cent. 



C«Hio0 4 (P0 4 Ca)2 + H 2 requires C = 16-59, H = 2-76, Ca = 1841, 

 P = 14-28 per cent. 



Source of the Hexosephosphate. — In order to show that this compound was 

 formed from the added phosphate, two quantities of 50 c.c. of yeast-juice 

 were incubated at 25°, one with 30 c.c. of a 30-per-cent. solution of glucose 

 and the other with 30 c.c. of a 0*3 molar solution of potassium phosphate 

 containing 30 per cent, glucose. After two hours both were boiled and 

 filtered, 30 c.c. of each filtrate were treated with the same quantity of 

 magnesium nitrate and potash to remove any free phosphate and, after 



