1911.] Immunisation by Means of Bacterial Endotoxins. 55 



Table VIII. 



Eeference No. of 



Amount of 



Amount of 





guinea-pig. 



endotoxin. 



culture. 



Result. 





m \ -o' 







10 





A loop 



Lived. 



12 



2-0 





7 



1 -o 







8 



1-0 







9 



1 -o 







13 



0-5 







14 



0-5 







15 



0-5 







3 



Control 





+ Dead, Dec. 13. 



4 









5 







Lived. 



The animals which survived were alive December 31, 1909. 

 On the whole, it would appear that the plague endotoxin does confer 

 considerable protection against living plague bacilli. 



Simultaneous Inoculation with Two and Three Different Endotoxins. 

 A few inoculations were simultaneously done with two and three different 

 endotoxins. 



The endotoxins were injected subcutaneously on August 11, and the 

 cultures were inoculated intra-peritoneally on September 15, i.e. five weeks 

 later. The typhoid endotoxin was prepared on June 29, the cholera endo- 

 toxin on June 29, and the diphtheria endotoxin on June 17. 



The results are given in the following tables (Tables IX and X). 



Series I. — Two endotoxins. 



Table IX. 



Eeference No. 

 of guinea-pig. 



Endotoxin, August 11. 



Culture, September 15. 



Result. 



1 { 



2 

 3 

 4 

 5 

 6 

 A 

 B 



"25 mgrm. typhoid 

 + - 25 „ cholera 



Control 



j- 1 loop cholera 

 1 



1 „ „ 

 0-5 „ typhoid 



'5 „ „ 

 0-5 „ 



1 „ cholera 



+ Dead, September 16. 

 Lived. 



+ Dead, September 16. 



C 

 D 

 E 

 F 





0-5 

 0-5 

 0-5 

 0-25 



, typhoid 



+ ,1 !> 



Ill, but recovered. 

 + Dead, September 16. 



