66 Method of Disintegrating Bacterial and other Organic Cells. 



micrograph of unground B. mycoides, e and / the same after grinding for 

 15 and 30 minutes respectively. 



III. Cream of Micrococcus pyogenes aureus. Amount = 3 c.c. Speed 

 1700 revolutions per minute. Number of living organisms estimated by 

 means of agar plates. 



Colonies on the Plates. 



Controls before 



After grinding. 



grinding. 



15 minutes. 



30 minutes. 



45 minutes. 



60 minutes. 



1/1,000,000 dil. 

 0-1 c.c. 

 2 colonies 



1/1,000,000 dil. 

 -1 c.c. 

 1 colony 



1/100,000 dil. 

 0-1 c.c. 

 13 colonies 



1/100,000 dil. 

 -1 c.c. 

 14 colonies 



1/10,000 dil. 

 0-1 c.c. 



nil 



1/1,000,000 dil. 

 -5 c.c. 

 42 colonies 



1/1,000,000 dil. 

 -5 c.c. 

 12 colonies 



1/100,000 dil. 

 -5 c.c. 

 33 colonies 



1/100,000 dil. 

 -5 c.c. 

 27 colonies 



1/10,000 dil. 



-5 c.c. 

 14 colonies 



1/1,000,000 dil. 

 1 c.c. 

 145 colonies 



1/1,000,000 dil. 

 1 -0 c.c. 

 72 colonies 



1/100,000 dil. 



1 -0 c.c. 

 41 colonies 



1/100,000 dil. 



1 -0 c.c. 

 24 colonies 



1/10,000 dil. 



1 -0 c.c. 

 27 colonies 



Average per Unit Volume. 



Control before 

 grinding. 



After grinding. 



15 minutes. j 30 minutes. 



45 minutes. 



60 minutes. 



83,000,000 



35,300,000 j 7,900,000 



7,270,000 | 275,000 



In Plate 1, g is a photomicrograph of unground M. pyogenes aureus, h and 

 i the same after grinding for 15 and 30 minutes respectively. 



Conclusion. 



We believe that the results of these experiments show that the apparatus 

 here described does efficiently disintegrate bacterial cells. The apparatus is 

 simple to manipulate, and, moreover, its design provides absolutely against the 

 escape of any of the contents in the process of grinding, a consideration of 

 great moment when dealing with pathogenic micro-organisms. 



