92 Dr. W. M. Bayliss. [Apr. 7, 



Table II. 



Concentration of 

 enzyme. 



Eeciprocal x 100 of time to effect change of 



800 at initial stage. 



500 in middle of 

 reaction. 



1 



2-7 1 





-695^ 





2 



5-27 





1-235 





4 



10-0 



>A 



1 -9 





5 



13 -35 





2 -08 





8 



22 -25 





2 -44 





Table III. 



Concentration 

 of enzyme. 



Reciprocal 



x 10 4 of time for change of 



350. 



725. 



900. 



40 



3330 



445 -0 



162-0 



20 



1540 -0 



133 -4 



44-4 



10 



1000-0 



45-9 



15 -35 



4 



250 -0 



9-52 





2 



133-4 



6 12 





1 



67 -0 



<3 55 





The letters with brackets refer to the similarly labelled curves of fig. 1. 



It will be seen from these numbers that there is no obvious relationship 

 between the activities of the various concentrations. 



In order to find out whether the simplest form of the adsorption 

 equation 



x = y 1 '", 



where x is the reciprocal of the time taken to effect a given change, and y 

 the concentration of the enzyme, will satisfy the experimental data, the most 

 direct way is to take the logarithmic form of the equation, viz. — 



log a; = - logy, 



and plot the values on logarithmic paper. If 1/n is constant, then the 

 values will lie on a straight line and the tangent of the angle made by this 

 line with the axis of abscissae will be the value of 1/n* 



Fig. 1 gives a few cases to illustrate how far the experimental results 

 agree with a simple exponential law. 



* See Freundlich, ' Zeitschr. f. physik. Chem.,' 1907, Band 57, p. 391 ; and Ostwald, 

 ' Lehrbuch der allgem. Chemie,' 1906, 2te AufL, Band 2, Teil 3, p. 232. 



