98 Mr. S. J. Meltzer. On the Distribution and [Apr. 11, 



and its substrate, preliminary to the particular chemical change brought 

 about by the enzyme in question. 



Adsorption between enzyme and substrate as affected by the presence of 

 neutral salts is investigated and found to follow the laws of "electrical" 

 adsorption. 



The relation between the concentration of an enzyme and its activity is 

 shown to be expressed by an exponential formula, the value of the exponent 

 varying considerably according to circumstances. In certain conditions it 

 may be unity and in others the square root, but is usually between the two. 



Accordingly, the view that the rate of an enzyme action at any given 

 moment is a function of the amount of the adsorption compound of enzyme 

 and substrate in existence at that time is to be regarded as fairly well 

 established. 



The expenses of the research were defrayed from a grant by the Govern- 

 ment Grant Committee. 



On the Distribution and Action of Soluble Substances in Frogs 

 Deprived of their Circulatory Apparatus. 

 By S. J. Meltzer, New York. 



(Communicated by Prof. A. E. Cushny, F.R.S. Received April 11, — 

 Read May 18, 1911.) 



(From the Department of Physiology and Pharmacology of the Rockefeller Institute 

 for Medical Research.) 



In view of the great distributory efficiency of the cardio-vascular 

 apparatus, no serious consideration has been given to the possibility of the 

 existence of other modes of distribution of material in the animal body. In 

 the following, results of experiments will be briefly presented which give 

 unmistakable evidence of an efficient distribution of substances in 

 cardiectomised frogs. 



In these experiments the heart was exposed, ligated, and removed, and the 

 incision closed again. Such a removal of the heart eliminates also the 

 activity of the lymph vessels and the lymph hearts which empty their 

 contents into veins. Injections were given into the various lymph sacs of 

 the body and into the abdominal cavity. The results to be reported were 



