1911.] Forms of Trypanosoma brucei (pecaudi), etc. 163 



space which was possibly surrounded by a limiting membrane. It was in 

 connection with this membrane (?) that the blepharoplast was found, hence 

 the use of the term "detached" (Plate 3, fig. 15). It was found that, as the 

 protoplasmic mass became larger, a clear area developed within it. This 

 increased in size, the nucleus of the mass became more distinct, and finally 

 the blepharoplast appeared gradually to approach the mass until it became 

 part of it. Such a movement on the part of the blepharoplast is well-nigh 

 inconceivable unless there was originally some connection between it and the 

 main mass of protoplasm. Such, however, I have never been able to 

 demonstrate. 



Eventually the spherical masses became ring-shaped, resembling in every 

 respect those met with in the red cells. 



These stages were always observed within the vacuoloid space, which 

 enlarged to accommodate the requirements of the developing trypanosome. 

 As in the red cells, forms almost mature were eventually found. These, 

 which possessed free extremities, often assumed S shapes within a faintly 

 defined capsule, the nucleus and blepharoplast being situated as in an 

 ordinary trypanosome. Later observations showed that these forms were 

 invariably found free in the plasma of the spleen, bone-marrow and axillary 

 gland several days previous to the appearance of the intra-corpuscular forms. 



Forms found in Lung Smears. 

 The probability of schizogony occurring in the lung as in the case of 

 Schizotrypanum cruzi was borne in mind, but no definite schizonts were seen 

 in any of the smears. Yet the forms figured by Chagas* and described by 

 him as parasites in the lung of vertebrates preparing for schizogony were 

 exceptionally numerous in the lung of a gerbil on the sixth day. In 

 fact they were the only forms present in smears from the lung at that time, 

 but no suggestion of merozoite formation was ever observed. Some forms 

 seen resembled the so-called latent bodies of Trypanosoma gambiense described 

 by Moore and Breinlj and more recently by FanthamJ as occurring both in 

 T. gambiense and T. rhodesiense infections. Their method of formation, 

 however, seems to be somewhat different to that described by these authors. 

 It is, perhaps, sufficiently indicated by the cycle of events shown in 

 Plate 3, figs. 19 — 32. In addition there was markedly evident the presence 



* Chagas, C, "Ueber eine Neue Trypanosomiasis des Menschen," 'Memorias do Instituto 

 Oswaldo Cruz,' August, 1908, vol. 1, No. 2. 



+ Moore, J. E. S., and Breinl, Anton, " The Cytology of the Trypanosomes," ' Annals 

 Tropical Med. and Parasitol.,' July, 1907, vol. 1. 



J Fantham, H. B., "The Life-history of Trypanosoma gambiense and Trypanosoma 

 rhodesiense as seen in Eats and Guinea-pigs," 'Roy. Soc. Proc.,' B, 1910, vol. 83. 



