164 Developmental Forms of Trypanosoma brucei (pecaudi), etc. 



of individual spherical forms dividing into two, and occasionally three, 

 separate bodies, each of which eventually assumes a trypanosome shape. 

 As a result, large numbers of what were undoubtedly very young 

 trypanosomes, each with a well-defined nucleus but without undulating 

 membrane and flagellum, were present in the lung smears. 



No definite intra-corpuscular or free encysted forms were ever seen in the 

 lung or peripheral blood. The former appeared exclusively in the spleen, 

 while the latter were found without fail in the spleen, bone-marrow and 

 axillary glands. 



The axillary gland showed an unending variety of young trypanosomes 

 corresponding in the main to those described above in the lung, while the 

 encysted stages were also very numerous. 



A marked feature, perhaps worthy of note, was the constant presence of 

 free chromatin granules in nearly all the smears from the second day 

 onwards. These were apparently derived from the nuclei of disintegrating 

 trypanosomes, and possibly represented a first stage in development. 



DESCEIPTION OF PLATE 3. 



Magnified 2000 diameters. Drawn with Zeiss Oc. No. 12, obj. oil imm. 2 mm., 

 and tube length 155. 



Fig. 1. — Long form with free flagellum. 



Fig. 2. — Stout granular form with very short flagellum. 



Figs. 3—13. — Intra-corpuscular cycle as seen in spleen on sixth day. 



Figs. 14 — 18. — Extra-corpuscular and apparently encysted forms in spleen, sixth day. 



No. 14 shows granules and very small bodies, the former possibly representing 



infective granules, the latter possibly a very early stage of development. 

 Figs. 19 — 32. — Forms found in lung smear on sixth day. 19 — 25 show possible formation 



of so-called " latent body " of Moore and Breinl. 26 — 32, metamorphosis of 



latent body into trypanosome. 



