1911.] Serum Reactions in Pulmonary Tuberculosis. 381 



two hours after the addition of corpuscles* and haemolysin. With this 

 technique I frequently noticed various rates of haemolysis taking place. The 

 protocol of November 27, 1910, with inhibitive serum 2225 (Table III) 

 shows the results that were obtained in this regard. However, this has no t 

 proved a practical method of observing the comparative activity of inhibitive 

 sera, partially because of the importance of the amount of complement 

 available in regard to time effects, and the great difficulty of maintaining in 

 complement a constant two-unit strength. 



In Table III are also given the results obtained by leaving antigen-inhibitive 

 serum mixtures in room temperature and in the cold. With these tubes the 

 addition of complement to the mixtures before or at the time of the use of 

 the indicators apparently makes no practical difference. 



These results lead to an attempt to dry rapidly antigen-inhibitive serum 

 mixtures, and then determine their effect upon complement as seen in 

 Table IV. Serum 2223 is from a patient which gave previously the 



Table IV.— Protocol 13.12.10. 







Haemolysis. 



S. 2223— 



3 squares macerated in 



6 



I -0 c.c 





Complement (2 units) 

 -5 c.c. 



Almost none. 

 None. 



S. 30 (dog)— 



3 squares macerated in 



6 



1 -0 c.c 





Complement (2 .units) 

 -5 c.c. 





10 











Antigen only — 



3 squares macerated in 



6 

 10 



I -0 c.c. 



saline 



Complement (2 units) 

 -5 c.c. 



Complete. 



S. 2223— 



3 squares macerated in 



10 



L -0 c.c. 









Almost none. 



S. 30 (dog)— 



3 squares macerated in 

 6 

 10 



•0 c.c. 



saline 





Complement added with 

 systemic couplet 



None. 



Antigen only — 



3 squares macerated in 

 6 

 10 



L -0 c.c. 



saline 







Complete. 

 Almost complete. 



* Hemolysin and corpuscles have been added separately, that is, previously sensitised 

 corpuscles have not been employed. 



2 F 2 



