388 Mr. A. H. Caulfeild. Inhibitive and Fixation [Aug. 24,. 



Table VIII.— 5.3.11. 

 From the previous protocol Sera 1372, 860, and Babbit 106 were classed 

 indifferent, the others gave evidence of varying amounts of inhibitin. 



Antigen. 



Sera. 



Haemolysis. 



Haemolysis of corpuscles 

 sensitised by adsorption. 





S. 1372, '3 c.c.* 



Almost none 



Almost complete. 



0-5 „ 





Some + 





S. 870, '3 c.c. 



Almost none + 



Some. 



0-5 " 





Almost complete 





10 „ 



S. 860, -3 c.c. 



None 



Almost complete. 



0-5 „ 







1-0 „ 



S. (Rab.) 106, -3 c c. 





Complete. 



0-5 „ 





Some 



1-0 „ 



S. 302, -3 c.c. 







0-5 „ 





Complete 





1-0 „ 



S. 1870, -3 c.c. 



Almost complete 





0-5 „ 





Complete 





1 -o „ 



Saline, 1 c.c. 



None 





0-5 „ 





Almost none + 





* "3 c.c. was obtained by using *5 c.c. of saline serum mixture containing approximately 

 '15 c.c. serum. 



(9) Variation in — 

 Unfortunately, it has not always been recorded in standardising comple- 

 ment whether this was from one or more animals. 



Lately, however, some endeavour has been made to note this, and, in 

 24 instances, when the complement was from only one animal, the 

 following standardisations were made : — 



1 unit strength in a 1/15 dilution or less in 4 guinea-pigs. 



1 „ 1/20 „ „ 11 



1 „ 1/40 „ „ 6 



1 „ 1/40 „ over 3 „ 



Total 24 „ 



This method of estimating the fixative power of complement is not 

 satisfactory, for in the few examples of sera which, in dilutions of 1/20,. 

 that did not completely hasmolyse the corpuscles, the 1/10 dilution had 

 a very rapid effect; and in this latter dilution seemed efficient in the 

 succeeding protocol, although, according to the method of standardisation, 

 did not contain two units. 



