410 



Messrs. L. Hill and M. Flack. 



[July 6, 



the wet tissues, and therefore to kill the bacilli a concentration must be used 

 which would also kill the tissues. 



One of the most potent methods of treatment is to draw blood in increased 

 volume to the infected part, by fomentations, blisters, etc., the blood itself 

 having bactericidal and immunising properties. We suggest that inhalation 

 of weak concentrations of ozone, by mildly irritating the respiratory tract, 

 may bring more blood to the part and thus have the curative effect of a 

 fomentation or blister. 



Fat has a power of absorbing ozone until it smells strongly of ozone, and it 

 retains the ozone for a long time. Mr. F. Kidd kindly tried for us the 

 application of ozonised lard or vaseline to foul chronic ulcers of the leg, but 

 found that while hot fomentations were efficacious in cleaning up and 

 rendering sweet control cases of ulcer, the ozonised ointment had little 

 effect. 



For the investigation of the respiratory metabolism we used the Haldane- 

 Pembrey gravimetric method. Mice or small rats were placed in a beaker fitted 

 with a thermometer and the beaker placed in a Hearson air-bath to keep the 

 external temperature constant. In a first series of experiments the ozone 

 was generated by a specially made small ozoniser, and led partly through the 

 animal chamber and partly through a collecting wash-bottle, in order that its 

 concentration might be determined. 



The water vapour given off by the animal varied so much with the passing 

 of urine and faeces, and possibly with the animal putting up its fur, 

 as it does when depressed by the ozone, that we can lay no weight on the 

 calculation of oxygen intake. The weakness of the gravimetric method lies 

 in the fact that the oxygen is calculated from the difference between loss of 

 body-weight and output of water and C0 2 , and not directly measured. 



We shall confine our considerations in these experiments to the loss of 

 body-weight, and the C0 2 output. Table II shows the loss of weight 

 sustained by the animal, the amounts of H 2 and C0 2 given off before, 

 during, and after ozone. It is seen that there is a marked depression during 

 and after the administration of ozone. The table gives a random selection 

 made from 25 similar experiments. 



The figures for the loss of weight represent the loss of weight of the animals 

 weighed in the respiration chamber. Evaporation of urine and faeces when 

 passed contribute to this loss. As the loss of body-weight is also influenced 

 by the passing and evaporation of the urine and water from the faeces, the 

 C0 2 output results are the more trustworthy. 



As the concentration of ozone given by the small generator seemed to be 

 too high, we obtained the ozone in the following experiments by generating it 



