1911.] Destiny of Cholesterol in the Animal Organism. 



463 



during the feeding period, as the following weighings show : December 28, 

 2-7 kgrm. ; December 31, 2*5 kgrm. ; January 1, 2"6 kgrm. ; January 7, 

 2*45 kgrm. ; January 11, 2 - 5 kgrm. ; January 20, 2*5 kgrm. ; January 23, 

 2 - 5 kgrm. ; January 30, 2-5 kgrm. ; February 1, 2 - 6 kgrm. When the 

 animal was killed the stomach was full. The liver weighed 115-45 grm., 

 and was unusually large for an animal of this weight, being 4 - 44 per cent, 

 of the body weight. The total cholesterol, free and combined, was found to 

 be 0-2425 grm., and the free cholesterol 0-2294 grm. The ester cholesterol 

 was therefore - 0131 grm. 



Experiment II. — This rabbit (B) was fed on cabbage stalks from 

 December 28, 1910, to February 16, 1911. It consumed 1-5 lbs. per day, 

 and on two occasions during the feeding period it was also given some 

 ether-extracted bran. The weight of the animal remained constant, the 

 following being the weights during the feeding : December 28, 2-4 kgrm. ; 

 December 31, 2'35 kgrm.; January 3, 2-5 kgrm.; January 7, 2-45 kgrm.; 

 January 11, 2-5 kgrm. ; January 20, 2 - 5 kgrm. ; January 23, 2 - 45 kgrm. ; 

 January 30, 2-3 kgrm. ; February 6, 2-4 kgrm. ; February 9, 2-4 kgrm. ; 

 February 11, 2 - 4 kgrm.; February 13, 2 - 34 kgrm.; February 16, 2*4 kgrm. 

 The animal was killed on February 16 and the stomach was full. The liver 

 weighed 82-91 grm., i.e., 345 per cent, of the body weight. The total 

 ■cholesterol, free and combined, was 0J932 grm., and the free cholesterol 

 01228 grm. The ester cholesterol was then 0-0704 grm. 



Cholesterol Content of the Livers of Rabbits fed on Bran which has been 

 thoroughly extracted with Ether to remove all Fat and Phytosterols. 



This diet was selected, as previous experiments had shown that rabbits 

 can be kept at constant weight for many days together on this food. As 

 the food was sterol-free, the influence of any absorption of vegetable sterols 

 on the cholesterol content of the livers was eliminated. 



Experiment III. — A healthy buck (C), weighing 2 - 3 kgrm., was fed on as 

 much bran as it would consume from April 1 to April 8, 1910. The weights 

 taken occasionally during the diet period were 2-3, 2-3, 2-2, 2-15, 2-2, 2-2, 

 2-2 kgrm. The liver weighed 62-2 grm., i.e., 2 - 83 per cent, of the body 

 weight. The total cholesterol, free and combined, was - 1596 grm., and 

 the free cholesterol 0-124 grm. The ester cholesterol was, therefore, 

 0-0356 grm. 



Experiment IV. — This was a large doe rabbit (D), weighing 3-1 kgrm. 

 It was fed from March 27 to April 11, 1911, and consumed during this 

 period 1160 grm. of the bran. The weights of the animal taken occasionally 

 were 3-1, 3'2, 31, 2-9, 2'9, 2-9, 34, 3'1 kgrm. The liver weighed 69-63 grm., 



