464 Messrs. Ellis and Gardner. Origin and [Nov. 8, 



or 2-25 per cent, of the body weight. The total cholesterol, free and 

 combined, was 0-232 grm., free cholesterol 01527 grm., and ester cholesterol 

 0-0793 grm. 



Cholesterol Content of the Liver of Babbits fed on Extracted Bran to 

 which Free Cholesterol had been added. 



It has already been proved that when cholesterol is given with the food of 

 rabbits, a portion only is excreted in the faeces, the remainder being absorbed 

 in the intestine, giving rise to a well-marked increase in the cholesterol 

 content of the blood. On the hypothesis mentioned at the beginning of the 

 paper we should expect to find in such animals an increase in the cholesterol 

 content of the liver. 



Experiment V. — A healthy rabbit (E), weighing 2 - 8 kgrrn., was fed from 

 March 27 to April 14, 1911, on extracted bran to which cholesterol was 

 added. It consumed during the period 1480 grm. of extracted bran, and 

 4 - 8 grm. of cholesterol. The cholesterol was given daily in 0-25 grm. 

 portions mixed with a small quantity of the moistened bran, and care was 

 taken that the animal ate the whole. The weights of the rabbit taken 

 occasionally were 2-8, 2-8, 2-8, 2-7, 2-7, 27, 2-8, 2"8, 2-6, 2-6 kgrrn. It thus 

 lost during the whole period - 2 kgrrn. 



The weight of the liver was 72-25 grm., i.e., 277 per cent, of body 

 weight. The liver contained 0-3315 grm. of cholesterol, and there was no 

 ester present. 



Experiment VI. — In this experiment a rabbit (F) was fed with as much 

 extracted bran as it would eat. On the 1st, 2nd, 4th, and 5th days it 

 received 0'25 grm. of cholesterol mixed with a little moist bran, and on the 

 6th, 7th, and 8th days - 5 grm. It thus had 2 - 5 grm. during the period. The 

 weights of the rabbit were as follows: 2-8, 27, 2-65, 2"6, 2-6, 2-65, and 

 2 - 7 kgrrn. The liver weighed 74*7 grm., i.e., 2*76 per cent, of body weight. 

 It contained - 341 grm. of free and combined cholesterol, 0-2144 of free 

 cholesterol, the ester cholesterol thus being - 1266. 



Experiments in which Babbits were fed on Extracted Bran, but the Cholesterol, 

 instead of being given by the Mouth, was injected in Olive Oil Solution 

 into the Peritoneal Cavity. 

 In order to ascertain whether cholesterin absorbed from other parts of 

 the body than the intestine would be carried to the liver and cause 

 an increase in the cholesterol content of that organ, two rabbits were 

 fed on extracted bran. In one, the control, pure olive oil was injected into 

 the peritoneal cavity, and in the other a solution of cholesterol in olive oil. 



