484 



Capt. A. D. Fraser and Dr. H. L. Duke. [Dec. 2, 



picric acid and 50 grra. sodium carbonate in 1 litre of water ; after draining 

 the paper, it is hung from a pin to dry until it is only just perceptibly moist ; 

 it is then cut up into f -inch lengths and stored in a closed tube. It is well 

 to keep a piece of the paper in each of the stock of tubes carried, so as to 

 make sure that hydrogen cyanide has not been stored up in the cork. 



City and Guilds College, 



South Kensington, London. 



Antelope Infected with Trypanosoma gambiense. 

 By Captain A. D. Fraser, E.A.M.C., and Dr. H. L. Duke. 



(Communicated by Sir John Bradford, Sec. B.S. Eeceived December 2, 1911, — 

 Bead January 25, 1912.) 



The Sleeping Sickness Commission of the Boyal Society, Uganda, 1908-10, 

 showed that water buck, bushbuck and reedbuck could be readily infected 

 with a human strain of Trypanosoma gambiense, and that clean laboratory- 

 bred G-lossina palpalis were capable of transmitting the virus from the 

 infected antelope to susceptible animals. 



In the present paper, observations which were made upon these antelope 

 during the eight months subsequent to the Commission's departure from 

 Uganda are recorded. Experiments are also described which show that 

 the duiker— another species of antelope common in most parts of Uganda — 

 can also be similarly infected with a human strain of T. gambiense. As 

 regards the antelope employed by the Commission, six of the nine remained 

 in apparently excellent health in April, 1911 — roughly, a year after they 

 were infected. 



Until Bushbuck 2428 escaped from the kraal, and Bushbuck 2372 died 

 338 days after its infection as the result of an accident, they had also been 

 healthy. A post-mortem examination was made immediately after death 

 in the case of Bushbuck 2372, but no evidence of trypanosomiasis was 

 found. 



Keedbuck 2427 appeared to be perfectly healthy for 200 days after it 

 had been infected. It then died suddenly. At the post-mortem examination 

 performed immediately after death the prescapular glands were found to 

 be the size of a hazel-nut. On section they were hsemorrhagic. There 

 were numerous petechias on the mucous membrane of the mesentery. The 



