1911.] Antelope Infected with Trypanosoma gambiense. 491 



Table V. — Giving Eesults of injecting the Blood of Antelope infected with 

 T. gambiense into susceptible Animals. 



Antelope. 



No. of 

 days after 

 infection 

 of 



antelope. 



u^d 



Quantity 

 of . m °°d 



Result. 



Remarks. 



Reedbuck2427 ... 



200 



Monkey 



2 



Inconclusive 



Monkey died. Negative 











for 9 days. 



Waterbuck 2378... 



306 



White rat 



1 







316 

 316 



Monkey 

 White rat 



5 







Bushbuck 2328 ... 



355 











2371 ... 



330 











2372 ... 



327 









Trypanosomes appeared in 













rat on the 12th day. 



Reedbuck 2357 ... 

 2359 



345 

 327 









2431 

 Bushbuck 2372 ... 



320 











338 



Monkey 





Inconclusive 



Monkey died before try- 

 panosomes couM have 

 appeared. 



Can a Duiker be Infected with a Human Strain of T. gambiense ? 



Experiment 99, Duiker. — On August 30, 1910, 3 c.e. of this buck's blood 

 were injected subcutaneously into a normal monkey to ascertain if the 

 antelope naturally harboured trypanosomes. The monkey's blood was 

 examined regularly for a month. No trypanosomes appeared in its blood, 

 the monkey remaining healthy. 



For nine days (January 25 to February 2, 1911, inclusive) laboratory-bred 

 G. palpalis known to be infected with a human strain of T. gambiense were fed 

 upon the buck. 



On February 4, the tenth day after the infected flies first fed upon the 

 antelope, T. gambiense appeared in fair numbers in its blood. 



On February It) and 11, 1911, 119 clean laboratory-bred G. palpalis were 

 fed upon the duiker. These flies were subsequently fed on a normal monkey, 

 which they infected after 28 days had elapsed from the date of their first 

 feed on the buck. Of 42 flies which were dissected, two were found to be 

 infected with flagellates. 



Remarks. — The duiker was free from trypanosomes inoculable into a 

 monkey on its arrival at the laboratory. 



T. gambiense appeared in the buck's blood on the tenth day after infected 

 flies had fed upon it, and clean laboratory-bred flies successfully transmitted 

 the infection to a healthy susceptible monkey. 



