-496 Dr. A. Harden and Mrs. D. Norris. Production of [Nov. 22, 



3. Results Obtained. 



The results obtained with the organisms employed are indicated in the 

 tables given below. The figures given in Columns 8 and 9 are the values 

 calculated from the actual results found for 10 grm. of sugar fermented. 



As previously mentioned, the results obtained with B. coli communis were 

 in every case negative. 



Table I. — Action of B. lactis aerogenes (Escherich) and B. cloacce (Jordan) on 



various Sugars. 



Experi- 

 ment. 



Organism. 



ugar. 



Weight 



lime 



C V 1 

 lOrin su^ar 



n l 



10 grm sugar 











growth. 



fermented. 



fermented. 







■ 



grm. 





grm. 



grm. 



1 



B. lactis aerogenes 



G-lucose 



4 



66 



3 weeks 



-ll 



1 -42 



2 

 3 





4 

 5 



85 

 00 





0-12 

 0-11 



1 -39 



4 





;; 



Fructose 



! 



47 





o-u 



1 -44 



5 



B. cloacx 





67 





0-11 



1-55 



6 





Mannose 



4 



15 





0-11 



1 51 



7 



8 



B. lactis aerogenes 



2 



: 



32 

 45 





o-u 



0-10 



1-36 

 1 "42 



9 





Galactose 





03 





0-06 



0-86 



10 







2 



40 





0-07 



0-85 



11 



B. cloacae 





4 



74 





0-08 



1 -41 



12 





Arabinose 



4 



38 





0-08 



1 -36 



13 



B. lactis aerogenes 



3 



92 





0-07 



1 -18 



14 



B. cloacse 





2 



39 





0-08 



1 -19 



15 





5 



00 





0-06 



1 -19 



16 







1 



83 





0-08 



1 -18 



17 



B. lactis aerogenes 



Isodulcite 



2 



03 



32 days 



0-43 



1 -45 



18 



B. cloacse 





1 



77 





0-67 



1 -54 



19 





2 



03 





0-04 



0-81 



It will be seen from the above table that glucose, fructose, and mannose 

 have given practically the same quantities of carbinol and glycol respectively, 

 whilst v/ith galactose the carbinol is slightly less in amount with both 

 organisms used, and the yield of glycol varies with the organism employed. 

 The results obtained in the case of arabinose show close agreement for both 

 organisms, although in all four cases lower figures are obtained than with 

 the hexoses. The question of isodulcite is interesting, as the amount of 

 carbinol obtained by means of B. lactis aerogenes is abnormally high com- 

 pared with that obtained from the other sugars or by using B. cloaca?. 



The galactose and arabinose used in the above experiments were previously 

 purified from traces of glucose by fermentation with yeast. 



The amount of glycol produced from glucose is decidedly lower than that 

 obtained by Harden and Walpole, a result which is possibly due to the 

 employment of a different strain of the organism. 



