540 Messrs. Twort and Ingram. Isolating and [Nov. 7, 



tubercular animals, and also with bovines suffering from pseudo-tubercular 

 enteritis. We believe this to be due, partly to the small amount of growth 

 in the fluid media, and partly to the fact that most of the growth was 

 obtained from solid media and therefore not made in the same manner as 

 diagnostic tuberculin. We also believe that a highly concentrated vaccine 

 will be required, and that we shall be able to prepare this now that one of 

 our strains of Johne's bacillus has started to grow on the surface of fluid 

 media containing the timothy-grass bacillus. 



In conclusion we may say that we are greatly indebted to Mr. de Vine, 

 Mr. Hamilton, and Dr. Bulloch for the specimens and materials they have 

 given us, to the donor of a naturally infected bull, and especially to the 

 Royal Society for the Government grants which enabled us to purchase the 

 other animals. An investigation such as this requires a large number of 

 bovine experiments, but these we have been unable to perform through lack 

 of funds. Insufficient apparatus in the earlier stages also caused us 

 cousiderable delay in the preparation of an efficient diagnostic vaccine, and 

 we had no animals on which to test the first vaccine prepared. We now 

 possess the necessary apparatus, and a number of vaccines are in course of 

 preparation, but unless the animals on which to test the vaccines are forth- 

 coming, we shall be unable to prove the efficacy or otherwise of the vaccines, 

 and shall be forced to leave it to other workers more favourably placed 

 financially. In any case it is to be hoped that other workers will test 

 a diagnostic vaccine prepared in the manner we have indicated, for the 

 economic loss from pseudo-tuberculous enteritis in such places as Denmark 

 and the Channel Islands is of a very serious nature. 



Some months ago we sent sub-cultures of the specific bacillus which we 

 had grown to various workers in the British Isles, France, and in Denmark, 

 and we hope that the desired vaccine will soon be obtained. 



BIBLIOGRAPHY. 



1. Johne and Frothingharn. " Bin eigentiimlicher Fall von Tuberkulose beim Binde," 



'Deutsche Zeitschrift fur Tiermedizin und vergleichende Pathologie,' 1895, 

 21 Bd., S. 438. 



2. Bieck. "Eigentiimliche Darmtuberkulose," 'Bericht iiber das % Veterina'rwesen im 



Konigreiche Sachsen fiir das Jahr 1896,' S. 118. 



3. D. van der Sluys. 4 Generalversammlung der niederlandischen Tierarzte in Utrecht,' 



26 u. 27 September, 1902. 



4. H. Markus. " Een specifieke darmontsteking bij het rund, waarschijnlijk van tuber- 



kuleusen aard," ' Tijdschrift voor Veeartsenijkunde,' 1903, Deel 30, Bldz. 193. 



5. H. Markus. "Eine spezifische Darmentziindung des Rindes waarscheinlich tuber- 



culbser Natur," ' Zeitschrift fur Tiermedizin,' 1904, 8 Bd., S. 68. 



