148 



Dr. W. J. Tulloch. The Isolation and 



55° C. was aimed at — and, usiug this serum to agglutinate laboratory strains 

 and strains isolated from wounds, the following results were obtained : — 



Diagram I. — Agglutination of Toxic Cultures with Serum of Animal 

 Inmumised with Culture A. 



Designation 



Source ^vhence culture was 



Dilutions of serum. 



JN ormai 

 serum. 



of culture. 



obtained. 



1/100. 



1/200. 



1/400. 



1/50. 



A 

 B 

 C 

 D 



Isolation from U.S.A. culture ... 

 "Whole culture, U.S.A. standard 



+ + 



+ + 

 + + 

 + + 

 + + 



+ + 

 + + 



+ + 



+ + 





E 





+ 



+ 



+ 





P 



Y 



+ + 



4 + 



+ + 





G- 



Y 



+ + 



+ + 



+ + 





H 



z 



+ + 



+ + 



+ + 





1 



Isolated from wound 



+ + 



+ + 



+ + 





2 













3 













4 













5 













6 













Control 



Bacillary suspension used for 

 immunisation. 



+ + 



+ + 



+ + 





Readings after 2 jiours at 55° C. 



+ + = complete floceulation, the supernatant fluid being clear. 



+ = agglutination obvious to the naked eye. 

 ( + ) = agglutination only in comparison with control tube of emulsion + 1/50 normal serum. 



It will be seen that only one of the toxic cultures — 1 — derived from a 

 wound agglutinates with this serum, while, on the contrary, all the laboratory 

 strains give a positive reaction. 



Culture 2 was therefore used for immunising a second animal, which 

 was bled out on attaining a titre of 1/800 when tested in presence of its 

 homologous organism. 



Using this serum, the results tabulated in Diagram II were obtained. 



It is therefore found that three of the organisms which failed to react in 

 presence of the agglutinating serum from the U.S.A. cultures are flocculated 

 by this serum. 



Culture 6 was used for immunising a third animal, and with this serum 

 the remaining bacilli 6 and 3 gave a positive reaction. 



