Serological Differentiation of Bacillus tetani. 149 



Dias;ram II. — Ago;lutiiiatiou of Toxic Cultures with Serum of Animal 

 Immunised with Culture 2. 



JJesignation 

 of culture. 



i 



»30urce Avhence culture "was 



Dilutions of serum. 



Normal 

 serum. 



obtained. 



1/100. 



1'200 





1 



A 



Isolation from U.S.A. culture ... 











B 

 C 

 D 



)) H )) 



Whole culture, L .b.A. standard 



— 



— 



_ 





E 



X , 











F 



T 











G 



Y 











H 



Z 











1 













2 





+ + 



+ + 



+ + 





3 













4 





+ + 



+ + 



+ + 





5 





+ 



+ 



— 





6 













Control 



Bacillarj suspension used for 

 immunisation 



+ + 



+ + 



+ + 





Eeadings after 2 lioui-s at 55° C. 



Diagram III. — Agglutination of Toxic Cultures with Serum of Animal 

 Immunised with Culture 6. 



Designation 

 of culture. 



Source whence culture Tvas 

 obtained. 



Dilutions of serum. 



Normal 

 serum. 



1/100. 



1/200. 



1/400. 



1/5 J. 



A 



Isolation from U.S.A. culture ... 











B 













C 



Whole culture, U.S.A. standard 











D 



Serum Institute X 





- 







E 



X 











P 



Y 











& 



Y 











H 



z 











, 1 



Isolated from "^vound 











2 













3 







+ + 



+ + 



+ + 





4 

 5 



:: ;: 











6 







+ + 



+ + 





Control 



Bacillary suspension used for 



+ + 



+ + 









immunisation 











Readings after 2 houi's at 55' C. 



