Serological Differentiation 0/ Bacillus tetani. 155 



Diagram VI. 



Designa- 

 tion of 

 culture. 



Culture to be 

 agglutinated was 

 grown in the selective 

 medium from : — 



Tetanus 



- 



igglutinating serum. 



1 



Non-toxic 



1 



agglutinating serum. 



Normal 



Type I. 



Tvne II 



Tvne ITT 



Pseudo T. 







serum. 



1/100. 



1/100. 



1/100. 



1/100. 



1/100. 



1/50. 





R27 



Brotli culture known 



+ + 



















to be toxic. 

















R62 





+ 















R68 





+ + 















R146 







+ + 













Controls 





+ + 



+ + 



+ + 



1 



-1- + 



-1- -1- 





Readings taken after 2 hours at 55° C. 



It is interesting to note that the non-toxic culture Tetanoid No. 1 was 

 isolated from the whole culture E 27; which as is seen from the above diagram 

 is a toxic tetanus bacillus of the U.S.A. type (Type I). The culture E 27 was 

 obtained from a man 49 days after the infliction of his wound. He had 

 received one dose of tetanus antitoxin on the day of wounding. Cultures E 62 

 and E 68, which are also of the U.S.A. type (Type I), were both obtained on 

 the 56th day after the infliction of the wound. In the case of one of 

 these, the man is not clear whether he had or had not received a dose 

 of antitoxin at the time of woundin<i. Culture 146 was obtained on the 

 8th day after wounding. A dose of antitoxin had been administered in this 

 case. Ee-examination of the wound made 30 days after the infliction of the 

 wound showed that no anaerobic organisms of any kind whatsoever were 

 present in the exudate. 



It is significant that three of these four toxic cultures, obtained from men 

 not showing evidence of tetanus intoxication, are of the U.S.A. type, 

 particularly in view of the fact that oi' the cultures obtained up to the 

 present from cases showing symptoms of tetanus occurring for the most 

 part in inoculated men, only one culture of tlie U.S.A. serological type 

 has been obtained. In my opinion this may mean that the prophylactic 

 inoculation of the antitoxin at present in use protects strongly against 

 intoxication from the U.S.A. type of bacillus, while its protection against the 

 other types is apparently short lived. 



VOL. xc. — h. 



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