Serological Differentiatiori^^Bs^iu^ tetani. 157 



The serum was considered to be absorbed if its titre was reduced to not 

 more than 1/100, from 1/400. 



The results were controlled by reactions with unabsorbed serum in the 

 same series of dilutions 1/100, 1/200, 1/400. 



As the following Table shows, the absorption of agglutinin test confirms the 

 previous observations and shows that considered as antigenics, the three types 

 are distinct from one another. 



Diagram YII. — Agglutinating Serum. 



Designation of culture. 



Unabsorbed serum — 

 Test bacillus 

 added. 



Absorbed serum — 

 Homologous bacillus 

 added. 



Absorbed serum — 

 Test bacillus 

 added. 



1/100. 



1/200. 



1/400. 



1/100. 



1/200. 



1,400. 



1/100. 



1/200. 



1/400. 







Type I 















^ (Type I) 



+ + 



+ + 



+ + 















C ,. 



2 (Tjpe II) 



+ + 



+ 



+ 



( + ) 



















+ + 



+ + 



+ + 









5 „ 









+ + 



+ + 



+ + 









3 (Type III) 









+ + 



+ + 



+ + 









6 „ 









+ + 



+ + 



+ + 













T 



ype II 















A 



_ 







+ 4 



+ + 



+ + 









C 









+ + 



+ + 



+ + 









2 



+ + 



+ + 



+ + 



( + ) 







( + ) 







5 



+ + 



+ + 



+ 















3 









+• + 



-H + 



+ 









6 









+ + 



+ + 



+ + 













Type III. 













A 









+ + 



+ + 



+ + 









C 









+ + 



+ + 



+ 









2 









+ + 



+ + 



+ + 









o 









+ + 





+ 









3 



+ + 



+ + 



+ 















6 



+ + 



+ + 



+ + 















YI. Are the Toxins Produced by eccch Type in any vjuy Specific, showing 

 Differences Corresponding to those made Manifest hy the Agglutination 

 Reaction in the Case of the Bacilli ? 



This question has not yet been gone into, but will form the subject of 

 further research, for the determination of such a specificity of toxin is of 

 paramount import in view of the influence it would have upon the prophylaxis 

 and therapeutics of the disease. 



VOL. xc. — B. 



o 



