The Production of Anthocyanins and Anthocyanidins. 265 



conditions as used in the examination of the yellow pigments. The ether 

 layer thus obtained, when shaken with dilute sodium carbonate solution, gave 

 no trace of green coloration. 



In the light of the results thus far obtained, the methods employed in 

 these preliminary investigations are capable of considerable simplification, 

 and by .taking advantage of this it is hoped that the isolation of both 

 myricetin and the sap pigment, which gives a yellow coloration with alkalis, 

 will be possible in sufficient quantities for confirmation of their identities by 

 means of derivatives. It is doubtful whether the quantities present will 

 allow of an insight into the nature of their glucosides being obtained, but an 

 attempt will be made if it appears reasonable. 



When time and circumstances permit, it is proposed to extend this work, 

 in the first place, to various other Violas, then to Eosa gallica (in which the 

 anthocyanin is cyanin), the Pelargonium zonule (in which the anthocyanin 

 is pelargonin), and other flowers, with a view to a systematic investigation 

 on the lines developed in the present paper, and in suitable cases the exami- 

 nation of the glucoside pigments with a view to discovering whether, where 

 the anthocyan pigments are accompanied by corresponding flavone deriva- 

 tives, the two classes of pigment occur attached to the same sugars. 



Having proved that the anthocyanin described in the present paper is 

 identical with "Willstatter and Weil's violanin, it is not the author's intention 

 to encroach upon the further work outlined by them {loc. cit.) concerning 

 the details of its constitution. 



VOL. XC. — B. 



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