Factor m the Mechanism of Bacterial Infection. 523 



At two different intervals of time — 2 and 24 hours afterwards — the mice 

 received injections of a suspension of Vibrion septique spores, half the 

 number of animals being injected subcutaneously at the same site (right 

 flank), the other half being injected subcutaneously on the back. The 

 results were as follows : — 



Gas Gangrene from Vibrim septique. Effect of separating Time and Site of 

 Injections of Bacteria and of Calcium Salt. 



Batch. 



No. of 

 mouse. 



Dose of Ca 

 nitrate. 



Interval of 

 time. 



Site. 



Kesult after 

 24 hours. 



A 



B 



C 

 D 



{l} 



1 6 J 



mgrm. 

 2 -5 



2 -5 



2-5 



2-5 



hours. 



li 

 li 



20 

 20 



Same 

 Different 



Same 

 Different 



+++000+++000 



' V ' y " V " ' 



B 

 P 



H 



{i} 



{i} 



5 

 5 

 5 

 5 



li 



li 



20 

 20 



Same 

 Different 



Same 

 Different 



f + 



( ^ 

 i + 



[ + 



f -t- 



•< + 



[ + 



f 

 \ 



I 



J 

 K 



I 27 J 



\ S I 



I 30 J 



10 

 10 



li 

 li 



Same 

 Different 



r + 

 < + 

 L + 



f -t- 



\ ^ 

 L 



L 



{11} 



10 



20 



Same 



{ : 



U 





10 



20 



Different 



{ 



Control 





Spores alone 







{ I 



This experiment shows that a minimal rupturing dose will elicit gas 

 gangrene in every case from the spores of Vibrion septique, even when the 



