Wounds of Men who received Prophylactic Inoculation. 541 



as possible after the addition of the kidney. This medium gives very heavy 

 growths of B. tetani, and appears to have some slight selective properties. 



Manipulatwfi Bov.ti'ne of tlie Cultures, 



Tubes A, B and C were examined at 2-day intervals. When tube C showed 



a growth consisting mairily of end-sporing bacilli, the culture was filtered 

 through a small piece of cotton wool to remove the kidney and sediment, 

 and then centrifuged, emulsified in saline, and tested by the agglutination 

 technique. 



Not infrequently culture C failed to show growth, although round end- 

 sporing baciUi might ultimately develop in tubes A or B. In such cases 

 A or B were heated 60° C. for 45 minutes and sub-cultures made into the 

 " selective " medium. In this way B. tetani was obtained from a number of 

 cultures which failed to show any growth when inoculated directly from the 

 swab into the " selective " medium. 



The technique of the agglutination test and the prepai'ation of agglutinating 

 sera are fully described in previous publications dealing with the serological 

 differentiation of B. tetani (1 and 2). 



BIBLIOGEAPHY. 



(1) Tulloch, ' Roy. Soc. Proc.,' B, voL 90, pp. 145-1.58. 



(2) Tulloch, 'Journal of E.A.M.C.,' Dec, 1917, p. 1. 



(3) Barber, 'Philippiiie Journ. of Science,' Med. Section B, August, 1914, vol. 2, 



p. 307. 



(4) Tarozzi, ' Cent. f. Bact.," Orig., 1905, vol. 38, p. 619. 



