Power of Crystalline Ovalbumin and Serum Albumin. 



35 



The second method involves the extraction of the serum by ether and the 

 crystallisation of the albumin by ammonium sulphate and acid at Pji 6'0 to 

 5 - ; 82 per cent, of the total albumin is thus obtained crystalline. After the 

 third crystallisation the specific rotation is constant, [«] D 18 = — 62"8° 

 [«] E 18 = -78-4°. 



The insoluble residue from the first crystallisation from natural serum is 

 shown to be a deposit of fatty material around the crystal surface. Its nature 

 varies with different preparations, sometimes phosphatides predominate : 

 sometimes cholesterol esters. The relation of fatty material and protein in 

 serum is discussed. 



In conclusion I wish to express my sincere thanks to Prof. F. Gowland 

 Hopkins for his valuable advise and criticism during the course of this 

 research. 



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D 2 



