88 



Mr. E. Ponder. The Hemolytic 



be observed correct to a few seconds, and readily reproduced with consistence. 

 In the case of sodium glycocholate acting in these high concentrations, a 

 variability in the time taken for haemolysis appears, even when factors such 

 as temperature are controlled. 



(2) The salt hemolyses more rapidly in dilutions of 1 in 1000 and there- 

 abouts, than in dilutions of 1 in 100. 



(o) A freshly prepared suspension of red cells seems to be less rapidly 

 hfcmolysed by the salt in all dilutions up to 1 in 1000, and especially in 

 dilutions of 1 in 100, and 1 in 50, than is a suspension which is 12 or 

 18 hours old. This is not unexpected ; the envelopes of these old cells 

 being probably weak. The importance of using a freshly prepared suspension 

 for quantitative haemolytic tests was insisted on in a previous communication. 



The following Table gives the times taken for complete haemolysis of 

 02 c.c. of standard blood suspension, freshly prepared, by various dilutions 

 of this salt, at 18° C. :— 



Table II. 



8. 



T. j 



5. 



T. 



50 



25 minutes. 



400 



40 minutes. 



100 



90 „ 



600 



30 



200 



65 „ 



1000 



10 „ 



It must be understood that this Table is merely representative of the 

 general behaviour of the salt, and that the times given are not the same for 

 all suspensions, but vary with the condition of the cells, etc. 



These results may be looked upon as unexpected — haemolysis occurring 

 more rapidly with a dilution of 1 in 1000 than with a dilution of 1 in 100. 

 It is obvious that the haemolysis depends on some factor other than the 

 simple solution of the lecithin and cholesterin envelope of the erythrocyte in 

 the solution of bile salt (2). It will further be observed that sodium 

 glycocholate is a feebly haemolytic agent compared with sodium taurocholate. 



It is with occurrences connected with the action of the glycocholate in 

 these relatively high concentrations that this paper is concerned. 



It is convenient here to insert a note regarding terminology. The 

 letter T is used to denote the time required for the complete haemolysis 

 of the amount of blood suspension used. The letter t represents the tem- 

 perature at which the experiment is conducted. The symbol 8 denotes the 

 number of cubic centimetres which contain 1 grm. of a haemolytic agent, in a 

 solution which is being used to produce haemolysis. For detail regarding 

 this nomenclature, the reader is referred to a previous paper (1) : in that 



