Action of Sodium Glycocholate. 



89 



paper the special technique employed in these hemolytic experiments is 

 given in full. In the case of sodium glycocholate, when working at tem- 

 peratures in the neighbourhood of 20° C, the exact temperature is very 

 important, as in this region a slight rise of temperature greatly accelerates 

 the haemolysis. Neglect of taking this into account is a fruitful source of 

 error. 



The Effect of Blood Serum on the Action of Bile Salts. 



Blood serum exerts a powerful inhibitory influence on the haemolytic 

 action of sodium glycocholate and sodium taurocholate. This is illustrated 

 by the following results, the experiment being carried out in the way 

 indicated in a previous paper (1), and in this case at 18° C. : — 



Table III. 





T. 



Sodium taurocholate, 1 in 1000 



„ „ "1 c.c. serum 

 Sodium glycocholate, 1 in 1000 



„ „ '1 c.c. serum 



3 minutes. 

 16 „ 

 10 „ 

 37 



With a view to discovering which constituent of serum produced the 

 inhibition, the inhibitory power, if any, of each constituent of serum was 

 studied. This problem will be dealt with later : the subject for consideration 

 at present being certain phenomena occurring when serum albumin is 

 brought into contact with sodium glycocholate and a blood suspension. The 

 fact that the bile salts cannot exert a haemolytic action in the presence of 

 blood serum is of great interest, since it throws new light on the con- 

 troversies regarding haemolysis and haemoglobinuria in jaundice (3). 



The Effect of Serum Albumin on the Hcemolytic Action of Sodium Glycocholate. 

 The following solutions are used : — 



(1) Solutions of sodium glycocholate in saline (0"95 per cent. XaCl). The 

 strength of these solutions is shown in the following Table : — 



Table IV. 



Glycocholate. 



Final concentration. 



Value of 8. 



per cent. 







2 5 



1 in ICO 



100 



1 25 



1 200 



200 



-833 



1 300 



300 



0-625 



1 400 



400 



0-5 



1 500 



500 



-417 



1 600 



600 



0-25 



1 1000 



1000 



