Action of Sodium Glycocholate. 



93 



sensitive and an insensitive suspension as regards the activity of either sodium 

 glycocholate or sodium taurocholate when acting upon it ; the difference 

 exists only towards the mixture of glycocholate and albumin. The above 

 mentioned phenomena occur not only with human erythrocytes, but with the 

 red cells of dogs, cats, rabbits and guinea-pigs. The length of time for which 

 a sensitive suspension remains unchanged varies. Some suspensions become 

 insensitive within half an hour of preparation ; others remain unchanged for 

 as long as 12 to 20 hours. 



Having considered the effect of varying the amount of glycocholate used 

 (Table V) when 04 c.c. of 1 per cent, albumin solution is used to accelerate 

 the haemolysis, it must now be considered what the effect is of varying the 

 amount of serum albumin. The following Table shows this. The substances 

 are mixed in the same way as that adopted for the drawing up of Table V, 

 i.e., by method 1 ; 04 c.c. of albumin is added in each case. 



Table VII. 



When r = 18. 



Albumin. 



G-lycocholate. 



1 in 100. 



1 in 400. 



1 in 600. 



1 in 1000. 



2 per cent. 

 1 



5 

 0-2 



30 seconds 

 28 minutes 

 43 „ 



56. „ 



20 seconds 

 30 „ 

 4 minutes 

 12 „ 



35 seconds 

 80 ,, 

 20 „ 

 1^ minutes 



5 minutes. 

 15 : , 

 35 



17 „ 



From a consideration of this Table it is obvious that the phenomena are 

 very complex. The subject will be left in the meantime, and referred to 

 again later (Table XIV). It will be sufficient to note here that the quantity 

 of serum albumin used in combination with various dilutions of glycocholate 

 is of the greatest importance. In the above experiment the suspension used 

 was an insensitive one. 



Certain of the occurrences met with when the sodium glycocholate, blood 

 cells, and albumin are mixed by method 2 may now be considered. 



In this method, - 4 c.c. of 1 per cent, solution of serum albumin is placed 

 in a tube, - 2 c.c. of standard suspension is added, and after five seconds, 

 - 4 c.c. of any desired concentration of sodium glycocholate is added. The 

 results of various dilutions of glycocholate may be given ; the suspension 

 used js sensitive, 



