94 Mr. E. Ponder. The Hemolytic 



Table VIII. 



When t = 18 



s. 



Method 1. 



Method 2. 



T. 



T. 



100 



5 seconds 



25 minutes. 



200 



10 „ 



30 seconds. 



300 



15 



20 „ 



400 



20 



25 „ 



600 



25 



40 „ 



1000 



2£ minutes 



4 minutes. 



The haemolysis does not occur so rapidly with method 2 as with method 1. 

 The difference is most marked when high concentrations of glycocholate are 

 used. In investigating the sensitiveness of a suspension, therefore, it is very 

 important that this difference be kept in mind. 



Tlw Protective Action of Serum Albumin. 



It has been seen that while serum albumin, if added to a cell suspension in 

 contact with sodium glycocholate, accelerates the haemolysis produced by the 

 latter, if it be added to a cell suspension it will protect it against the action 

 of the sodium glycocholate and serum albumin mixture. 



To investigate this further, one may put up four tubes, as follows : — 



Tube 1. — 1 c.c. suspension 0*2 c.c. of 1 per cent, serum albumin. 



Tube 2. — 1 c.c. suspension 0*1 c.c. of 1 per cent, serum albumin. 



Tube -3. — 1 c.c. suspension OOo c.c. of 1 per cent, serum albumin. 



Tube 4. — 1. c.c. suspension 0'02 c.c. of 1 per cent, serum albumin. 



The suspension used is a sensitive one. Allow all tubes to stand for 

 5 minutes. Examine the suspensions to see if they are sensitive or insensitive. 

 The following result is typical : — 



Table IX. 



When t = 18 ; 8 = 100 ; +0'4 c.c. 1 per cent, albumin. 



Tube. 



T. 



Tube. 



T 



Control 



15 seconds. 



3 



5 minutes. 



1 



40 minutes. 



4 



1 minute. 



2 



22 „ 







The effect, then, of adding a small quantity of serum albumin solution to a 

 sensitive suspension is to render it insensitive, The degree of insensitiveness 



