96 Mr. E. Ponder. The Hamolytic 



Results for four rabbits may be recorded : — 



Table XI. 



When t = 18. 



Babbit, 



T, 1st sample. 



T, 2nd sample. 



Albumin injected. 



1 



15 seconds 



24 minutes 



'1 gramme. 



2 



20 „ 



15 „ 



-04 „ 



3 



15 „ 



23 „ 



-08 „ 



4 



30 „ 



18 



-04 „ 



The injection seems to cause no untoward effect on the rabbit, which is 

 under chloroform anaesthesia. 



These phenomena will be found to be not peculiar to serum albumin : 

 other similar substances act as powerful accelerators of glycocholate haemo- 

 lysis and as protective agents, when used differently, just like serum albumin. 

 Peptone is such a substance : its actions are exactly parallel with those of 

 the albumin. On trying the effects of various animal extracts, similar 

 properties were found to be possessed by both adrenalin and pituitrin (Parke, 

 Davis preparations). Since it has been shown that pituitrin contains 

 histamine, this at once suggests the possibility of the phenomena being due 

 to histamine or histidine, appearing in both the albumin, the peptone, and the 

 pituitrin (5). 



The Effect of Histamine on Glycocholate Haemolysis. 



A series of solutions of histamine (Burroughs Wellcome) is prepared, the 

 following dilutions being convenient : — 1 in 500, 1 in 1000, 1 in 2000, 

 1 in 5000, 1 in 8000, and 1 in 10,000. These solutions are made in normal 

 saline (0 - 95 per cent, sodium chloride). 



Histamine and histidine are, per se, non-haamolytic. If - 4 c.c. of 2 - 5 per 

 cent, glycocholate solution have 0'2 c.c. of standard blood suspension added, 

 and if, after 5 seconds, - 4 c.c. of 1 in 1000 histamine be added, haemolysis 

 occurs instantaneously. 



The histamine thus has the accelerating action found with the serum 

 albumin, whose action was probably due to the histamine contained in it as 

 an unavoidable impurity. This is a most interesting fact, for it enables 

 many more exact observations to be made on the phenomena mentioned 

 above in connection with serum albumin. Certain preliminary notes must 

 first be made. 



The Effect of Histamine on Colloid Gold. 



In view of the fact that sodium glycocholate protects a gold sol, it is 

 important to know the action of histamine on such a colloid. Histamine 



