98 



Mr. E. Ponder. The Hemolytic 



Table XII. 



When t = 18. 



s. 



Albumin. 







Histamine. 



albumin. 



A llrnlinp 



. Lift, it llllC 



albumin. 



■ 



histamine. 



Allrfi hip 



. Li ft. i 1 I 1 I J V 



histamine. 



100 



65 mins. 



95 mins. 



43 mins. 



105 mins. 



55 mins. 



110 mins. 



200 



40 „ 



30 „ 



3 



76 „ 



40 sees. 



73 „ 



300 



9 „ 



90 sees. 



30 sees. 



58 „ 



30 „ 



50 ., 



400 



1 niin. 



40 „ 



20 „ 



35 „ 



15 „ 



15 „ 



500 



50 sees. 



30 „ 



30 „ 



18 „ 



30 „ 



4 „ 



600 



1 min. 



35 ,, 



50 „ 



10 „ 



50 „ 



45 sees. 



1000 



5 mins. 



3 rams. 



3^ mins. 



23 „ 



2£ mins. 



li mins. 



This Table, which is representative of the general results obtained with an 

 inactive suspension, expresses several important points : — 



(1) Histamine behaves similarly to the serum albumin, as an accelerator of 

 the glycocholate haemolysis. 



(2) The rendering of the haemolysing solution acid causes haemolysis to be 

 more rapid: if the haemolysing solution be alkaline, the haemolysis is 

 retarded. The amount of deviation from neutrality is very small in the 

 above case. 



(3) The relation between the speed of haemolysis under the various 

 conditions and the amount of glycocholate used is expressed. Columns 1 

 and 2 are confirmatory of the Tables illustrating the behaviour of an 

 inactive suspension. 



The question of the reaction of the haemolysing fluid is obviously one of 

 great importance. A series of observations in which the Pn is determined 

 would be ideal : the difficulties attendant upon the use of buffer solutions in 

 connection with these haemolytic experiments are at present, however, 

 insuperable. The question is being investigated. 



It now remains to consider the effect of varying the quantities of 

 histamine employed for accelerating the glycocholate in its haemolytic 

 action. This is done in the following two Tables, the first Table illustrating 

 the results when a sensitive suspension is used, the second illustrating the 

 results in the case of an insensitive suspension. In each case, the tem- 

 perature at which the experiments were conducted was 18° C. : the substances 

 were mixed by method 1. 



