Action of Sodium Glycocholate. 



103 



4. Blood cells which are rapidly lueinolysed by glycocholate and histamine 

 become insensitive on standing, but to a less degree with an old suspension 

 than with a freshly prepared one. 



5. Serum albumin and peptone solutions, and also pituitrin, produce both 

 the rapid hsemolysis when mixed with sodium glycocholate, and also the 

 protective effect when added to blood cells. This latter occurs in vivo as 

 well as in vitro. These occurrences may be due to the presence of histamine 

 or allied substances. 



6. Several facts suggest that these phenomena are mainly due to dis- 

 turbance of surface tension, similar to those which are met with in colloidal 

 solutions. They cannot be explained by the theory that the bile salt 

 -dissolves the corpuscle envelope. 



7. Suspensions of cells which are derived from blood drawn into a fluid 

 which prevents coagulation behave differently from suspensions of cells 

 which are derived from blood drawn into a fluid which permits coagulation 

 to occur. 



8. A protection against hsemolysis by the histamine-glycocholate mixture 

 is also conferred by blood serum. 



9. The presence of blood serum inhibits the hsemolytic action of sodium 

 taurocholate and sodium glycocholate. 



REFERENCES. 



1. Ponder, E., " A Method for investigating the Hsemolytic Activity of Chemical 



Substances," 'Roy. Soc. Proc.,' B, vol. 92 (1921). 



2. Schafer, 'Text-book of Microscopic Anatomy,' p. 370 (1912). 



3. Von Noorden, ' Metabolism and Practical Medicine,' vol. 2, p. 260 (1907). 



4. Muller, P., ' Serodiagnostic Methods ' (Transl. Whitman), p. 20 (1913). 



5. Abel and Kubota, ' Jour. Pharm. and Exp. Ther.,' vol. 13, p. 243 (1919). 



6. Bayliss, W. M., 'Nature of Enzyme Action,' pp. 22 et seq. (1914). 



