the Optical Rotation of the Proteins of Blood Sera. 191 



Table VIII. 



Protein and 

 variety. 



Section. 



Position. 



0. 



1 



1. 



2. 1 



4. j 



6. 



8. 



9. 



10. 









Adopted wave-lengths. 







Pseudo-globulin — 























7 



2790 



2847 



2860 



2875 



2900 



2930 



2935 



2948 





t 



2790 



2735 



2710 



2670 



2630 



2590 



2562 



2520 





I 



2410 



2415 



2420 



2430 



2445 



2460 



2475 



2520 





y 



2790 



2845 



2858 



2864 



2895 



2929 



2935 



2943 





6 



2790 



2744 



2710 



2686 



2635 



2595 



2572 



2521 





I 



2418 



2423 



2428 



2430 



2445 



2461 



2480 



2521 



Eu-globulin — 





















Horse 



y 



2775 



2825 



2849 



2865 



2885 



2905 



2920 



2925 





e 



2775 



2740 



2718 



2675 



2635 



2579 



2559 



2532 





I 



2420 



2435 



2437 



2443 



2460 



2480 



2490 



2532 





y 



2795 



2830 



2850 



2864 



2880 



2905 



2920 



2930 





e 



2795 



2760 



2730 



2680 



2648 



2605 



2578 



2530 





I 



2422 



2424 



2426 



2437 



2450 



2469 



2480 



2530 



Albumin — 























y 



2785 



2840 



2952 



2863 



2870 



2880 



2885 



2897 





t 



2785 



2730 



2720 



2688 



2660 



2620 



2592 



2545 





I 



2456 



2457 



2460 



2465 



2475 



2490 



2508 



2545 





y 



2783 



2815 



2825 



2850 



2871 



2880 



2882 



2885 





£ 



2783 



2755 



2730 



2685 



2645 



2610 



2575 



2540 







2450 



2455 



2457 



2465 



2480 



2495 



2510 



2540 



The Characteristics of the Mean Curves. 



The curves are divided into Sections a, (5, j, 8, e, £ as before (loc. cit.). 

 Among the more remarkable characteristics are the curious form of the head, 

 Section S, and the step-like prominences or " steps " to which reference was 

 made in the first paper. It was hoped that the analysis of the serum into its 

 several proteins would have revealed the origin of these steps by showing 

 them to be irregularities due to imperfect superposition of the bands of two 

 or more proteins, but that expectation has not materialised. On the other 

 hand, these characteristics appear in both the globulins with unerring 

 regularity, and with but little variation, in either magnitude or position. In 

 albumin the " steps " are not so numerous, and they are not much in evidence 

 above the tenth position. Below that two large " steps " are evident. 



It is difficult to say whether their positions, that is, their extinction 

 coefficients, are precisely the same for all specimens of a given protein or not. 

 They repeat themselves with sufficient regularity to suggest that the positions 

 may be definite approximately as shown ; but on the other hand, the variation 

 is too great to attribute it to experimental conditions. The general conclusion 



