266 Misses M. Stephenson and M. D. Whetham. 



Phosphorus Estimation. 



Weight of " phosphatide " fraction 

 taken ; in grm. 



Mgrm. of 

 phosphorus found. 



Percentage of 

 phosphorus. 



-0280 

 -0284 

 -0240 

 '0241 



-346 

 0-382 

 0-270 

 0-270 



1 23 

 1 -34 

 1 12 

 1 12 



Nitrogen Estimation. 



Weight of lipoid taken ; in grm. 



Mgrm. of 

 nitrogen found. 



Percentage of 

 nitrogen. 



0-1126 ("fat") 



-1260 (" phosphatide ") 



0-989 

 823 



0-78 

 0-73 



Probably both fractions are complex mixtures of lipoid bodies similar to 

 those obtained from the tubercle bacillus [Goris, Goris and Liot, 1920 (1)]. 

 The two are treated separately in this research on account of the absence of 

 phosphorus in fraction A and its presence in fraction B. 



Analysis of the Medium. 



As described above, the contents of one Eoux bottle (150 c.c. of original 

 medium) were made up to 250 c.c, from which the agglutinated bacteria and 

 solid mineral matter (chalk, etc.), were then removed by filtration. The 

 filtrate was then analysed for glucose or other carbon compound present. 

 Glucose estimations were carried out on 5 c.c. of the diluted medium by the 

 method of Wood-Ost [Wood and Berry, 1903, (4)]. Ammonia was estimated in 

 the first and last samples by the method of Van Slyke [1911, (3)], in order 

 to ensure that growth of the bacteria had not ceased through lack of 

 nitrogen. 



Experiment 1. — Growth of the Bacillus in respect of Total Nitrogen and Lipoids 

 on the usual Inorganic Medium, containing as well 1 per cent. Glucose and 

 lper cent. Acetic Acid, added as Sodium Acetate. 



Twenty-five Eoux bottles, prepared and inoculated as described above, 

 were incubated at 37° and bottles were withdrawn at intervals in sets of two, 

 one for the estimation of total nitrogen, and the other for the estimation of 

 lipoids and of the organic constituents of the medium. The disappearance of 

 acetic acid was not followed stage by stage in this first experiment, but it 



