Studies in Fat Metabolism of Timothy Grass Bacillus. 267 



was ascertained that by the time the glucose had been utilised the acetic acid 

 had also disappeared. 



The reaction of the medium was kept constant by chalk, which was spread 

 over the bottom of the Roux bottles ; the Ph at the beginning, end, and all 

 intermediate stages remained 8'0. This was tested by phenol red, and kindly 

 checked for us by the hydrogen electrode by Miss Jordan Lloyd. 



The figures given in column 1 of the following table represent the actual 

 amounts obtained from single Eoux bottles (150 c.c. each), which were of 

 necessity the units employed ; column II represents the same amounts 

 calculated for 100 c.c. medium, from which the points on Curve 1 are taken. 

 AVhere estimations were made on duplicate Eoux bottles, the figures are 

 bracketed. 



Fat Synthesised. 



Da}- of 

 experi- 

 ment. 



" Fat " fraction. 

 Weight in grin. 



" Phosphatide " 



fraction. 

 Weight in grm. 



Total lipoids. 

 Weight in grm. 

















r. 



II. 



I. 



II. 



I. 



II. 



7* 

 14 



21 



22 



28 



36 



60 



-0054 

 -0198 

 0-0399 \ 

 0-0348 J 

 Organic cons 

 0-0179 1 

 0-0268 J 

 -0145 

 0-0089 \ 

 0-0095 J 



-0036 

 0132 



0288 



tituents of th 



O 0149 



-0096 



O -0061 



-0083 

 0181 

 0-0284 1 

 O-0280 J 

 e medium co 

 0-0240 \ 

 0-0241 J 

 -0230 

 0-0162 1 

 0-0152 J 



0-0054 

 0120 



-0188 



mpletely utili 



0160 



-0152 



-0105 



0137 

 -0379 

 0623 1 

 0-0625 J 

 sed. 



0-0419 1 

 0501 J 

 -0375 

 0-0251 1 

 0247 J 



-0090 

 -0250 



-0420 



-0310 

 0-0124 

 -0170 



* The results obtained on the seventh day are too small relatively to the experimental error 

 to he important. They merely serve to indicate that growth had begun. 



Protein Nitrogen Synthesised. 



Day of 

 experiment. 



Grm. per 

 Eoux bottle. 

 I. 



Grm. per 

 100 c.c. medium. 

 II. 



7 



21 



28 

 36 



0-005 

 027 "I 

 -027 J 

 0-027 

 0-026 



0-003 



0-018 



018 

 0-017 



u 2 



