352 Mr. C. H. Browning and others. Relationships between 



On analysis : 



0130 grm. gave 14-5 c.c. N at 15° and 757 mm. N = 13 per cent. 



Calculated for C 21 H 19 N 4 OCl2 ; N = 13 4 per cent. 

 The compound has therefore the following formula : — 



CH 



N 



C 6 H 5 NH.CO.CH 2 CI 



[45.] 2'7 .Diaminoacridine Chloropropionate was prepared like the chlor- 

 acetate and was purified by crystallisation from concentrated hydrochloric 

 acid. 



[48.] 2.7.Diamino-3.6.dimethi/lacridine (Acridine yellow) was prepared 

 according to the method of Ullmann and Naef* and Ullmann and Marie.f 

 To purify it, it was ground while moist and warmed on the water-bath with 

 sufficient sodium hydroxide to render the mixture alkaline ; after cooling it 

 was filtered, washed and pressed down. The moist precipitate was then 

 dissolved in a small quantity of glacial acetic acid and whilst hot a little 

 cone, hydrochloric added gradually until the solution changed to a deep red, 

 when water was added till turbid and cooled. The hydrochloride separates in 

 fine prismatic, deep orange or red crystals which dissolve in water with a green 

 fluorescence. 



[49.] 2.7. Diamino-3.6.dimethylacridine methochloride. — The hydrochloride 

 prepared as above was precipitated as the base with sodium hydroxide, 

 filtered, washed and thoroughly dried in a vacuum desiccator and then 

 converted into the diacetyl derivative. 36 grms. of the base were mixed with 

 13 grms. of acetic anhydride in a small flask furnished with an air condenser 

 and boiled gently for \ hour. The substance gradually dissolved. It was 

 diluted with water, and to the cooled solution just sufficient ammonia was 

 added to decompose the anhydride and precipitate the acetyl derivative. 

 After filtering, washing and drying it was extracted with a little absolute 

 alcohol, which removes a small quantity of a pale yellow substance. 1 grm. 

 of the acetyl derivative was dissolved in 10 grms. of nitrobenzene, heated to 

 170° and 8 grm. of methyl sulphate added. On cooling, the mixture 

 solidifies to a crystalline mass which was filtered and washed free from nitro- 

 benzene with ether. The crystalline residue was boiled with cone, hydro- 

 chloric acid for J hour, when nearly the whole dissolved with a deep red 



* 'Ber.,' vol. 33, p. 913 (1900). 

 t 'Ber.,' vol. 34, p. 4308 (1908). 



