420 Dr. C. Shearer. Heat Production and Oxidation 



flasks sunk in the water of the thermostat, h for the first flask was 0238, 

 and for the second h = 0'0236. Temperature adjusted all round to within 

 a hundredth of a degree to 14'5° C. Cooled sperm added and readings 

 started. 



Time 3.30 P.M. galvanometer scale reading 



„ 6.30 „ „ „ 17 mm. E flask containing eggs. 



„ 9-30 „ „ „ 26 „ 



„ 12.30 A.M. „ „ 47 „ 



„ 2.30 „ „ „ 59 „ ,, „ „ 



At end of experiment 95-100 per cent, of eggs were in healthy free- 

 swimming early gastrula stages. Kjeldahl determination gave 146"2 mgrm. 

 of egg nitrogen present in flask contents. The heat liberated in first hour 

 6'34 grm.-calories, in the fifth hour 28 grm.-calories, and in the eleventh hour 

 74 - 4 grm.-calories (see Curve, fig. 2. a). 



7U. u g.cals. in 11 tvrs. 

 / 



/ 



/ 



5 10 Time hTS » 



Fig. 2, a.— Curve of heat production to Experiment II. 146"2 mgrm. egg nitrogen. 



(h) Oxygen and CO2 Determination. — 2 c.c. of same lot of egg material as 

 that put in the flask in the first part of the experiment, placed in a chamber 



