76 



Notes on Toxoplasma gondii. 



plentiful reagents and apparatus for my work there. I should also like to 

 thank the Trench military authorities for their courtesy and kindness during 

 my stay at the Matmata Fort, where they gave me all possible assistance.] 



EXPLANATION OF PLATE 9. 



Unless otherwise stated, the preparations were stained with iron haematoxylin and 

 counterstained with licht-grun and picric acid. 



Fig. 1. — Mononuclear leucocyte enclosing several toxoplasnis. x 2000. 



Fig. 2. — Living specimen drawn at intervals of 5 or 10 minutes, x 2000. a, b, still 



attached to cellular debris ; c-e, after becoming free. 

 Fig. 3. — Detached endothelial cell containing two parasites, x 1000. 

 Fig. 4. — Detached endothelial cell with two nuclei and several parasites, some of which 



are being set free, x 1000. 

 Fig. 5. — Three endothelial cells of the serous membrane, x 1000. a, normal cell ; 



b, cell with two parasites and nucleolus already beginning to break up ; c, cell 



with a further degenerated nucleus, and several rapidly dividing parasites 



forming a mass at s. 



Fig. 6. — Cell from the serous membrane of the omentum with more than 60 parasites, 



some forming a compact mass at s. x 1000. 

 Fig. 7. — A toxoplasm from the liver of Mouse H, showing chromatoid masses in its 



cytoplasm, x 2000. Stained Giemsa. 

 Fig. 8. — Free dividing form from liver, x 3000. Stained Giemsa. 



Figs. 9 and 10. — Living specimens from peritoneal fluid with one or more refringent 

 granules in cytoplasm, x 2000. 



Fig. 11. — Living specimen with two nuclei from peritoneal fluid, x 2000. 



Fig. 12. — Intracellular parasites from a transverse section of omentum, x 3000. Stained 

 iron hematoxylin, eosin, and licht-grun. 



Fig. 13. — Form with some peripheral chromatin in the nucleus, x 3000. Stained iron 

 hematoxylin and orange G. 



Figs. 14 and 15. — Recently-divided forms with extranuclear chromatoid masses probably 

 consisting of volutin, x 2000. 14. Stained iron hematoxylin and fuchsin S. 



Figs. 16-25.- — Free parasites showing binary fission, x 3000. 25. Stained iron haema- 

 toxylin and orange G. 



REFERENCES. 



1. Bass, C. G, and Johns, F. M. "The Cultivation of Malaria Plasmodia ... in vitro," 



'Amer. Journ. Exp. Med.,' October, 1912, No. 4, vol. 16, pp. 567-579. 



2. Chatton, Edouard. " Protozoaires Parasites des Branchies des Labres," ' Archives 



de Zool. Experimental et Generale,' 5th ser., vol. 5, p. 254. 



3. Nicolle, C, and Manceaux, L. " Sur une Infection a Corps de Leishman (ou 



Organismes Voisins) du Gondi," ' Coinpt. Rend.,' October, 1908. 



4. Nicolle, C, and Manceaux, L. " Sur un Protozoaire Nouveau du Gondi," 'Coinpt. 



Rend.,' February, 1909. 



5. Nicolle, C, and Manceaux, L. " Sur un Protozoaire Nouveau du Gondi {Toxo- 



plasma n. gen.)," ' Archives de lTnstitut Pasteur de Tunis,' May, 1909, pp. 97-103. 



6. Nicolle, C, and Conor, M. " La Toxoplasmose du Gondi," ' Bull. Soc. Path. Exot.,' 



March 12th, 1913, vol. 6, No. 3. 



