86 Groivth and Sporulation of Tertian Malarial Parasites. 



Joukoff, N. M. (1913). "Culture du Parasite de la Malaria," 'Compt. Eend. Soc. Biol.,' 



vol. 74, No. 3, pp. 136-138. 

 Marchiafava, E., Bignamr, A., and Mannaberg (1894). ' Two Monographs on Malaria 



and the Parasites of Malarial Fever.' (1) By Marchiafava and Bignaini ; 



(2) by Mannaberg. 

 Ross, Sir Ronald (1910). ' The Prevention of Malaria,' p. 89. 



Rowley Lawson, Mary (1913). " The Extra-cellular Relation of the Malarial Parasite to 

 the Red Corpuscle and its Method of Securing Attachment to the External 

 Surface of the Red Corpuscle," ' Journ. Exp. Med.,' vol. 17, No. 3, pp. 324-335, 

 with six plates. 



Stephens, J. W. W., and Christophers, S. R, (1908). 'The Practical Study of Malaria,' 

 p. 34. 



Thomson, J. G., and McLellan, S. W. (1912). " The Cultivation of One Generation of 

 Malarial Parasites (Plasmodium falciparum) in vitro, by Bass's Method," ' Ann. 

 Trop. Med. and Parasitol.,' vol. 6, pp. 449-462, with two plates. 



Thomson, J. G, and Thomson, D. (1913). " The Cultivation of One Generation of 

 Benign Tertian Malarial Parasites (Plasmodium vivax) in vitro, by Bass's 

 Method," 'Ann. Trop. Med. and Parasitol.,' vol. 7, No. 1, March, pp. 153-165, 

 with one plate. 



Thomson, J. G., and Sinton, J. A. (1912). "The Morphology of Trypanosoma 

 gambiense and Trypanosoma rhodesiense in Cultures ; and a Comparison with the 

 Developmental Forms described in Glossina palpalis," ' Ann. Trop. Med. and 

 Parasitol.,' vol. 6, No. 3, pp. 331-357, with three plates. 



EXPLANATION OF PLATE 10. 



All the figures in this plate represent the growth of the parasites in the culture tube. 

 Magnification 1600 diameters. 



Fig. 1 is a malignant tertian parasite at the time of inoculation of the culture tube. 



Corpuscle is shrunken ; no pigment is seen, and no stippling of the corpuscle. 

 Fig. 2 represents 12 hours' growth at 36° C. No pigment is yet visible. 

 Fig. 3 represents 23 hours' growth at 41° C. Pigment is not jet evident, but the 



parasite has increased in size. 

 Figs. 4 and 5 represent 36 hours' growth ; note the appearance of a compact mass of 



pigment. Fig. 5 is a double parasite. 

 Figs. 6 and 7 show commencing segmentation after 47 hours' incubation. Fig. 6 shows 



5 daughter-cells, and fig. 7 20 spores. 

 Figs. 8, 9, and 10 show sporulating forms after 51 hours' incubation. 



Fig. 11 shows complete sporulation (32 spores) after 52 hours' incubation. The containing 



corpuscle has burst and liberated the spores. 

 Fig. 12 was obtained after 56 hours' incubation. 



Fig. 13 represents a young merozoite of the second generation which has entered a new 

 corpuscle, after 75 hours' incubation ; note that there is no vacuole. The 

 parasite is only 1"5 n in its longest diameter. 



Fig. 14 represents sporulation of the second generation after 3 days' incubation. 



Fig. 15 shows a young merozoite of the third generation after 4j days' incubation. 



Fig. 16 represents sporulation in the third generation after 6 days' incubation ; only 

 8 spores have formed. 



Fig. 17 is a young merozoite of the fourth generation after 7 days' incubation. 



