214 Messrs. G. Graham and E. P. Poulton. The Alleged 



and the creatinine was then determined by Folin's method and by the 

 distillation method. The creatine was then converted into creatinine by 

 heating on the water-bath for three and a half hours or longer with 5 c.c. 

 normal hydrochloric acid, in order to get the creatinine -I- creatine figure. 

 These experiments show that in no case was any creatine converted into 

 creatinine by the distillation, even .when (Experiment 5) there was actually 

 more creatine (0152 grm.) than creatinine (0T19 grm.) in the urine 

 (Table V). In the first three experiments, when creatine was present in 

 small amounts, practically all the creatine added to the urine was converted 

 into creatinine by heating for three and a half hours on the water-bath. In 

 the fourth experiment, when 0*076 grm. of creatine was present in the urine, 

 only 48 per cent, of the creatine was converted into creatinine after 

 three and a half hours, and even after five and a half hours only 89 per 

 cent, could be recovered. In the fifth experiment, with a very large amount 

 of creatine (0152 grm. per 100 c.c), only 28 per cent, was converted after 

 heating for three and a half hours. 



Table V shows that Creatine is not broken down into Creatinine by Heating 

 with Phosphoric Acid in the Distillation Method. 





Amount of 



Creatinine. 



Creatinine 



Creatine 







No. of 



creatine 

 calculated 







+ 



creatine 



recovered 

 in the 



Creatine 

 added to 



Per- 









centage 

 recovered. 



expt. 



as 



creatinine 



Folin's 

 method. 



Distilla- 



calculated 



estimation 



urine as 





added to 

 urine. 



tion 

 method. 



as 



creatinine. 



as 



creatinine. 



creatinine. 





grm. per 



grm. per 



grm. per 



grm. per 



grm. per 



grm. per 







100 c.c. 



100 c.c. 



100 c.c. 



100 c.c. 



100 c.c. 



100 c.c. 





] 



o-oi 



0-071 



0-071 



-081f 



o-oi 



o-oi 



100 



2 



0-016 



0-095 



0-096 



o -not 



0-015 



0-016 



95 



3 



0-034 



0-112 



0-113* 



145f 

 f 0-154f 



033 

 -037t 1 



0-034 



97 

 f 47-5 



4 



0-076 



0-117 



0-117 



\ 0-185J 

 [ 0-183§ 



-068t \ 

 0-063§ J 



0-076 



\ 89-5 

 I 83 



5 



0-152 



0-119 



0-120 



0-1631- 



0-044 



0-152 



24 



* Distilled 1| hours. 



f Heated for 3£ hours on the water-bath. 



» &h a )> 



§ By the autoclave method. 



Benedict and Myers (3) have also noticed that the water-bath method 

 gave an incomplete result, when urine containing - 066 grm. creatine per 

 100 c.c. was used. Mellanby (18) has pointed out that five hours' heating 

 on the water-bath is usually necessary to get a complete conversion. 

 However, our observations show that three and a half hours on the water- 



