Excretion of Creatine in Carbohydrate Starvation. 217 



true creatinine and creatinine + creatine was still 1*72 grm. The apparent 

 creatine had, therefore, increased to 031 grm., while as a matter of fact 

 no true creatine was excreted. 



In Experiment II (G. G-.) f pint of cream and two eggs were eaten on each 

 day. The amount of aceto-acetic acid excreted was greater than in Experi- 

 ment I, and the nitroprusside reaction was quite strong on the first day, 

 0'3 grm. being excreted. On the second and third days the aceto-acetic 

 acid amounted to 1'06 and 1'46 grm. The true creatinine output was 

 slightly lower than in the case of E. P. P., but it remained equally constant 



Table VII.— Experiment I. Subject, E. P. P. Date, June 18-20, 1913. 



Day. 



Volume. 



Total 

 nitrogen. 



By the Folin method. 



After removal of 

 aceto-acetic acid. 



Aceto-acetic acid. 



Apparent 

 creatinine. 



Creatinine 

 + 



creatine. 



Apparent creatine. 



True 

 creatinine. 



True 

 creatine. 



Grm. per 

 day. 



Concentra- 

 tion in 

 100 cc. 



Grm. per 

 day. 



G-rin. per 

 100 cc. 



Grrm. per 

 day. 



Grrm. per 

 day. 



2 

 3 



cc. 

 740 

 670 



1070 



grm. 

 12 



13 -o 

 16-25 



grm. 

 1 -82 

 1 -58 

 1 "42 



grm. 

 1 '80 

 1 -82 

 1 -72 







-24 

 0-30 







-018* 

 -028 



1-78 

 1 -81 

 1 -72 





 







1 



-872 j -065* 

 -874 j -081 



Diet eaten. — Day 1 and 2 : Cream, 300 cc Calorie value (approximate), 1060. 



Day 3 : Cream, 300 cc ; plasmon, 50 grm. ; eggs, 2. Calorie value (approximate), 1640. 



* On this day the volume of urine was small and an equal volume of water was added to it before the creatinine 

 determinations were made in order to get a reading on the colorimeter scale within the limits advised by Folin. This 

 dilution will halve the concentration of the aceto-acetic acid in the solution used for the Folin estimation, which 

 becomes actually less than that of the succeeding day. 



Table VIII.— Experiment II. Subject, G. G. Date, June 22-25, 1913. 



Day. 



Volume. 



Total 

 nitrogen. 



By the Folin method. 



After removal 

 of aceto-acetic acid. 



Acfto-acetic acid. 



Apparent 

 creatinine. 



Creatinine 

 + 



creatine. 



Apparenl 



Grm. per 

 day. 



, creatine. 



Grm. per 

 100 cc. 



True 

 creatinine. 



True- 

 creatine. 



Grm. per 

 day. 



Concen- 

 tration per 

 100 cc 



1 

 2 

 3 



cc 

 1310 

 1235 

 1310 



grm. 



12 -4 

 11 "86 

 Diet ( 



grm. grm. 

 1 -46 1 53 -07 

 1 -21 1 -43 -22 

 1 17 1-53 0-36 

 iaten. — Cream, 400 cc ; eggs, 2. 



-0053 

 -0175 

 -0274 



Calorie val 



grm. grm. 

 1 -52 

 1 -43 

 1-52 



ae (approximate), 1400 



0-3 

 1 -06 

 1 -46 



0-029 

 0-085 

 0-112 



