218 Messrs. G. Graham and E. P. Poulton. The Alleged 

 Table IX.— Experiment III. Subject, M. D. Date, June 25-28, 1913. 



Day. 



Volume. 



Total 

 nitrogen. 



■ 



By the Folin method. 



After removal of 

 aceto-acetic acid. 



Aceto-acetic acid. 



Apparent 

 creatinine. 



Creatinine 

 + 



creatine. 



Apparent 



G-rm. 

 per day. 



creatine. 



Grm. per 

 100 c.c. 



True 

 creatinine. 



True 

 creatine. 



Grm. 

 per day. 



Concentra- 

 tion per 

 100 c.c. 



1 

 2 



3 



c.c' 

 600 

 750 

 1220 



grm. 

 11-99 

 14-43 

 14-82 



Diet eate 



grm. 



1 -93 

 2-02 



2 -09 



a. — Cream, 



grm. 

 2-05 

 2-14 

 2 -25 



500 c.c. ; eg 



0-12 

 0-12 

 016 



»s, 3. Cal 



o-oio 



0-008 

 srie value 1 



2 -03 

 2-15 

 2 -27 



approximate), 1600 ca 



0-43 

 99 



ories. 



-036* 

 -066* 



* On these days the volume of urine was small and an equal volume of water was added to it before the creatinine 

 determinations were made in order to get a reading on the colorimeter scale within the limits advised by Folin. This 

 dilution will halve the concentration of the aceto-acetic acid in the urine. 



throughout the experiment, viz., 1*5 grm. On the first day the apparent 

 creatine was already - 07 grm., and on the second and third days it had risen 

 to - 22 and 36 grm. respectively, but no true creatine was excreted at all. 

 The difference in the scale reading between the apparent and true creatinine 

 was 2 mm. on the third day of this experiment. It has been previously 

 shown (p. 211, fig. 1) that the error in the estimation of the creatinine caused 

 by the aceto-acetic acid in these two experiments agrees fairly closely with 

 the error caused by adding the same concentration of a sodium aceto-acetate 

 solution to normal urine. 



As it was necessary to be absolutely certain that if any creatine was 

 present in the urine it would be converted into creatinine by the methods 

 we have used, some pure creatine was added to a part of the urine of the 

 third day in Experiment II. The estimation of the creatinine + creatine in 

 the plain urine and in the urine to which creatine had been added was 

 carried out under precisely similar conditions on the same water-bath. 

 The result (Table VI) showed that in the plain urine no creatine was 

 converted into creatinine, but that the creatine was almost quantitatively 

 converted into creatinine in the sample of urine to which creatine had been 

 added. This control experiment shows that creatine if present in the urine 

 is detected and estimated by the methods employed. 



As we wished to confirm the results of these experiments on ourselves, 

 Dr. M. Donaldson very kindly took the following diet for three days, viz., 

 f pint of cream and three eggs each day. We wish to express our thanks 

 to him. The urine gave Eothera's nitroprusside test on the first day, and 

 this reaction was well marked on the second and third days. The true 



