Destiny of Cholesterol in the Animal Organism. 231 



replaced by ordinary hard-boiled egg, pounded up and moistened with warm 

 water. 



Diet (2) was replaced by the white of egg, and the yolk which had been 

 extracted with ether. 



Diet (3) was replaced by white of egg and extracted yolk to which about 

 2 per cent, of cholesterol had been added. 



Pens A and B. — Ten chickens were placed in pen A, weighing altogether 

 336 grm. After one week the weight of these chicks was 512 grm. ; 

 10 chickens were placed in B, weighing altogether 346 grm., and after one 

 week their combined weight was 555 grm. 



After one week two chickens were taken from pen A and three from 

 pen B, their total weight being 2604 grm. These were killed and analysed in 

 the usual way, the total ether extract being then made up to 1 litre ; 100 c.c. 

 of this extract yielded by direct precipitation 0-228 grm of compound, corre- 

 sponding to 0-0554 grm. of cholesterol, and after saponification 100 c.c. yielded 

 0-2908 grm. of the compound, corresponding to 0-0706 grm. of cholesterol. 



After 14 Days. — The eight chickens in pen A weighed 559 grm., and the 

 seven in pen B weighed 515*3 grm. From pen A three chickens weighing 

 205'5 grm. were taken, and from pen B two chickens weighing 150 grm., 

 their total weight being 355 - 5 grm. These were killed and analysed with 

 the following results : — The extract was made up to 1 litre ; 100 c.c. of this 

 gave by direct precipitation 0271 grm. of compound, corresponding to 

 0'066 grm. of cholesterol, while after saponification 100 c.c. gave - 365 grm. 

 of compound, corresponding to 0-0887 grm. of cholesterol. 



After 21 Days. — The five chickens in pen A now weighed 402 - 9 grm., and 

 the five in B weighed 496'6 grm. From pen A two chickens were taken, 

 weighing 1994 grm., and from B two chickens weighing 152*5 grm., their 

 total weight being 351'6 grm. These were killed and analysed in the 

 usual manner, and the ethereal extract made up to 1 litre ; 100 c.c. of this 

 extract gave by direct precipitation - 207 grm. of the compound, corre- 

 sponding to 0-0505 grm. of cholesterol, whilst after saponification 100 c.c. 

 gave - 382 grm. of the compound, corresponding to 0'0829 grm. of cholesterol. 



After One Month. — The three chickens in pen A now weighed 287"5 grm., 

 and the three in pen B 361 grm. Two chickens were now taken from pen A, 

 weighing 207 - 7 grm., and one chicken from pen B, weighing 103 - 5 grm., their 

 total weight being 311-2 grm. These were killed and analysed in the usual 

 manner, and ethereal extract was made up to 1 litre ; 100 c.c. of this extract 

 were taken, and by direct precipitation gave - 2 grm. of the compound, corre- 

 sponding to 0-0487 grm. of cholesterol, whilst 100 c.c. after saponification 

 gave - 2386 grm. of the compound, corresponding to - 058 grm. of cholesterol. 



