232 Messrs. J. A. Gardner and P. E. Lander. Origin and 



In Pen C. — Fourteen chicks were in this pen, weighing altogether 766 grm. 

 One of these died on the seventh day. Five chickens, including the dead 

 one, were now taken and analysed in the usual way, their combined weight 

 being 250T grm. The ethereal extract was made up to 1 litre, and the 

 following results were obtained : — 100 c.c. of the solution gave by direct 

 precipitation 0*2767 grm. of the compound, corresponding to 0551 grm. of 

 cholesterol, whilst after saponification 100 c.c. gave 0*3032 grm. of the com- 

 pound, corresponding to 0'0737 grm. of cholesterol. 



After 14 Days. — There were now nine chickens in pen C, weighing 

 657*5 grm. ; four of these, weighing 295*6 grm., were killed and analysed, 

 the ethereal extract being made up to 1 litre ; 100 c.c. of this extract gave 

 by direct precipitation 0*1646 grm. of compound, corresponding to 0*0400 grm. 

 of cholesterol, while 100 c.c. after saponification gave 0*2341 grm. of com- 

 pound, corresponding to 0*0569 grm. of cholesterol. 



After 21 Days. — Pen C contained five chickens, weighing altogether 

 514-8 grm. None were killed this week, and 



After 28 days the weight of the chickens was 614 - 5 grm. Three of these, 

 weighing 357*7 grm., were killed and analysed, and the ethereal extract 

 made up to 1 litre ; 100 c.c. were taken, and by direct precipitation gave 

 0*216 grm. of compound, corresponding to 0*0525 grm. of cholesterol, and 

 after saponification 100 c.c. gave 0*254 grm. of compound, corresponding to 

 0*0618 grm. of cholesterol. 



Pen D. — Fourteen chickens, weighing 532*2 grm., were in this pen to start 

 with. 



After 7 days the weight of the chickens was 742*8 grm. Five chickens, 

 weighing 249*8 grm., were killed and analysed with the following results : — 

 The ethereal extract was made up to 1 litre ; 100 c.c. of this extract gave 

 by direct precipitation 0*272 grm. of compound, corresponding to 0*0661 grm. 

 of cholesterol, while after saponification 100 c.c. of the extract gave 0*447 grm. 

 of the compound, corresponding to 0*1097 grm. of cholesterol. 



After 14 days the nine remaining chickens weighed 587*3 grm. ; four of 

 these, weighing 262 grm., were now killed and analysed with the following 

 results : — The extract was made up to 1 litre, and 100 c.c. gave by direct 

 precipitation 2432 grm. of compound, corresponding to 0*0511 grm. of 

 cholesterol, while 100 c.c, after saponification, gave 0*4919 grm. of compound, 

 corresponding to 0*1195 grm. of cholesterol. 



After 21 days the five remaining chicks weighed 447*3 grm., aud 



After 28 days their weight was 583*5 grm. ; three of these, weighing 

 352*5 grm., were now killed and analysed with the following results : — The 

 extract was made up to 1 litre, 100 c.c. of this gave, by direct precipitation 



