238 Messrs. W. E. Bullock and W. Cramer. 



Analysis of Lipoids of Transplantable Tumours. 





Carcinoma of 

 mouse. 



Carcinoma of mouse. Rapid growth. 



Sarcoma of rat. 

 Rapid growth. 

 No necrosis. 





Slow growth. No 

 necrosis. 



Healthy part. 



Necrotic part. 





Dry 



tissue. 



Total 

 Lipoids. 



Dry 

 tissue. 



Total 

 Lipoids. 



Dry 



tissue. 



Total 

 Lipoids. 



Dry 

 tissue. 



Total 

 Lipoids. 



Total lipoids 



per cent. 

 13 -3 



per cent. 



per cent. 

 13 -1 



per cent. 



per cent. 

 41 -1 



per cent. 



per cent. 

 18 '5 



per cent. 



Cholesterin 



1 -1 



8-8 



Trace 







Trace 







1 4 



7-8 



Cholesterin esters 







0-88 

 



11 -32 







6 6 

 

 84 6 







1 -3 

 

 11 -8 







9-7 





 90 -3 







2-54 

 Trace ? 

 38 -56 







6 2 

 

 93 8 







7- 2 

 1-9 



8- 







38 -8 

 10 -1 

 43 -3 



A comparison of the slowly growing mouse tumour with the healthy parts 

 of the rapidly growing mouse tumour shows that (1) the percentage of total 

 lipoids is practically the same ; (2) cholesterin forms 1 per cent, of the total 

 solids of the slowly growing tumour, while it is present only in traces in the 

 rapidly growing tumour ; (3) phosphatides form only a small proportion of 

 both tumours, though they are more abundant in the rapidly growing tumour ; 

 (4) both tumours are devoid of cerebrosides and cholesterin esters ; and (5), 

 that the bulk of the total lipoids of these two tumours consists of ordinary fats. 



The change in composition brought about by necrosis is shown by a 

 comparison of the figures for the healthy and necrotic parts of the rapidly 

 growing tumour. The most conspicuous feature of the change is the increase 

 in total lipoids, which are trebled in amount, the increase consisting to a great 

 extent of ordinary fat, and to a less extent of phosphatides. A trace of a 

 reducing substance is liberated by acid hydrolysis from the lipoids of the 

 necrotic parts, but whether this can be taken as an indication of the formation 

 of small amounts of cerebrosides during necrosis must for the present 

 remain doubtful. 



It is of particular interest to note that different strains of mammary mouse 

 carcinoma may show such wide variations in chemical composition, as is shown 

 here between the relative proportion of different lipoids, and more especially 

 of cholesterin. It has been pointed out in numerous papers from the 

 Imperial Cancer Eesearch Fund that marked biological differences exist 

 between the cells of different tumour-strains, even although these cells may 

 originally be derived from the same tissue (mamma). It is interesting to note 

 that there exist also chemical differences between cells derived from the same 

 tissue. 



