Salicin Hydrolysis by Enzyme Action. 



251 



parable among each other, are required. This can only be achieved by 

 varying the concentration of the enzyme at the same time as that of the 

 substrate, for, ceteris paribus, the extent of an enzyme action is, as shown by 

 the activity curves of figs. 1 and 3, determined by the proportion of enzyme 

 to substrate present in the reaction mixture. The case which constitutes the 

 fifth stage of the inquiry will now be considered. 



Table V. 



Temperatures at the 

 beginning and end 

 of each experiment. 



Salicin hydrolysed per cent, with the following molecular con- 

 centrations of the substrate and giro, of the enzyme per cm. 3 . 



M/5. 

 31 x lO" 5 . 



M/10. 

 13 5 x lO"" 5 . 



M/15. 

 8-7 x 10-s. 



M/30. 

 5-7 x 10" 5 . 



M/50. 

 5 x 10" 5 . 



8-0 



. 







17-2 









9 -0- 8 -3 







17'3 











9 -5- 9 -0 



_ 









18 -8 







17 -8-17 -5 



35 -0 













18 -1-18 -2 







34 -2 











18 -2-18 -4 



















39 -7 





18 -4 





















41 '9 



18 "7-18 -5 















40-3 







18 -8-18 -7 











36 -3 









23 -1-22 -5 



46 -1 













24 -9-25 -1 



















50 -5 





26 -1-26 -2 











53 3 









26 -2-26 -4 















50 -3 





61 -1 



27 -0 







56 -9 











29 -7 



62 -2 













30 -0-3C-5 







— 



65-7 







30 -5 











68 -6 



69-4 



31 -8-31 -6 







63 "7 









33 o-33 -8 





69 -4 



65 -8 









33 -8 









70 -0 





70 -0 



34 7-34 -8 











71 '7 





35 6-35 -7 







65-8 









36 -0 





69 -4 











36 -6 









66 -3 







39 -4-39 -9 











59 7 





41 -8^12 -0 



60-5 



58 -3 











42 -5-43 -0 









56-3 







43 













53 



43 6-43 o 







51 -2 









44-0-44 -4 











47 





47 "8-48 -0 





43 -3 











48 -0-48 -5 









37 -7 







48 -5 







37 -7 







36 4 



49 -0-49 -o 













34 -1 





50 2 



44-6 













51 -0 





35-6 











51 7-51 -5 







30 -7 









53 -5 









22 -7 







53 -5-53 -8 











22 5 



22 -4 



54 6 



33 -6 













57-5-57 -4 



23 7 













