SaMcm Hydrolysis by Enzyme Action. 

 Table VI. 



253 





Salicin hydrolysed per cent, with the following enzyme concentrations 



Temperatures at the 







per 



cm. 3 . 







beginning and end 

 of each experiment. 





















11-7 x 10-\ 







i-8 x iq~ 5 . 



3-3 x 10- 5 . 



6*8 x lO" 5 . 



15 x 10-\ 



18 x 10-\ 







8 -5- 8 -3 







— 



— 



— 



43-7 





9 -5- 8 -5 























48 -7 



10 -0-10 -5 



— 



16 











17-5 



— 



— 







— 



70-0 





17 -8-17 -9 



— 



24 -7 











18 -2-18 



— 



_ 











— 



78 -7 



18 -7-18 6 



— 



— 



— 



63 







19 -0 



18-3 













19 -5-19 -0 



— 





47-7 









20 -3-20 1 



— 



















84-0 



21 ■ 5-21 -1 



— 



— 



51 -6 < 









22 -5 



— 



— 















92 -0 



24 "4. 















78 -0 







24 -8-24 -9 



— 







61 -7 









25 -5-25 -6 



22 5 













25 -8-25 -6 



— 



38 -0 











26 -2 



— 















90 -0 





30 -0 



— 















95 3 





30 -2 



25 -2 



46 -8 











30 -4-31 -6 



— 











91 -0 







31 -1 



— 



















99 -0 



31 -6-31 -7 



— 







76 -0 









32 -9-33 -1 



— 









94 -7 







33 5 







46 -8 











98-0 





34 -0 



25 -2 













37 6-37 '8 



— 







74 -2 









40 -0-39 -9 



— 



















99 -7 



40 -2 



23 -0 













40-5 



— 



43 -3 







85 -0 







40-7 



' 















99 -0 





41 -8-42 -0 







65 3 









45 -0-45 -2 





35 -8 





73 3 



84 -0 





45 -0-45 -4 



I D .a 

 Lri n 













49 -1-49 -0 



13 -5 













50 "5-50 "4 











61 -3 



69-0 



51 1-51 









47-7 







52 -2-51 -6 













60 -7 



53 -6-53 -5 







26 -8 







54 -0 





14 -6 







42 -7' 





54 3 









35 







55 -0 













44-3 



56 -0 



6 -5 













57 







18 



■ 









Turning now to the last stage of the inquiry, the case of the substrate 

 concentration remaining constant while that of the enzyme changes, it 

 constitutes the study, properly speaking, of the influence of the enzyme 

 concentration on the optimum temperature. Although rendered unnecessary 

 by what precedes, the study is given in order to complete the present 

 investigation. For this a M/30 dilution of the substrate was chosen and 



VOL. LXXXVII. — B. T 



