Phenomena of " Clot " Formations. 371 



Table II — continued. 



Per cent, 

 (vol.). 



Grm. 

 mol. per 

 litre. 



Clotting 

 time, 

 sees. 



Per cent, 

 (vol.). 



Grm. 

 mol. per 

 litre. 



Clotting 

 time, 

 sees. 



Per cent, 

 (vol.). 



Grm. 

 mol. per 

 litre. 



Clotting 

 time, 

 sees. 



Ethyl Carbaminate. 



Propyl Carbaminate. 



Witte's Peptone. 



Per cent. 



















(weight) . 



















1 -25 



0-140 



26 



1 -25 



0-121 



36 



1 875 





213 



1-875 



-210 



38 



1-875 



0-182 



93 



2-5 





360 



2-5 



281 



56 



2 -1875 



212 



248 



3-125 





570 



3-75 



0-421 



147 



2-5 



-242 



360 



3 75 





1530 



4-875 



0-491 



296 



3 125 



0-303 



910 









5-0 



0-561 



564 











Phenol. 





5 625 



0-632 



750 













6-25 



0-702 



1448 









-625 





165 















-9375 





550 



Chloroform. 



A solution of water saturated with chloroform at 17° contains - 710 per 

 cent. When 2 - 5 c.c. of this solution was mixed with 05 c.c. N. calcium 

 chloride and 1 c.c. 4-per-cent. cholate solution, the clotting time was not 

 appreciably longer than when no chloroform was present. By diminishing 

 the concentration of the calcium salt to one half, the clotting time was 

 230 seconds in the presence of saturated chloroform water (2 - 75 c.c. in 4 

 = 0*041 grm. mol. per litre), as compared with 45 seconds, the clotting time 

 in the absence of chloroform. The clotting time in presence of - 064 grm. 

 mol. per litre amyl alcohol, and the same amount of calcium salt, was 

 155 seconds, and of - 038 grm. mol. per litre chloral hydrate 194 seconds. 

 The inhibitory action of chloroform is therefore greater than that of amyl 

 alcohol. 



Nitromethane. 



The action of nitromethane is anomalous. In the presence of 3 - 75 per 

 cent, the clotting time is 23 seconds, in the presence of 5 per cent, it is 

 57 seconds, and of 6-25 per cent, it is 42 seconds. It appears to behave more 

 or less like an acid, for in the presence of hydrochloric acid in N/800 con- 

 centration, the clotting time of cholate solutions is 41 seconds, in N/400 it 

 is 104 seconds, 3N/800 it is 185 seconds, and in N/200 it is 74 seconds. At 

 the highest of these concentrations the acid is sufficient to cause precipitation 

 of free cholalic acid ; on keeping at 50° the precipitate disappears and a gel 

 then forms. The lower concentrations produce no separation of free organic 

 acid in form visible to the naked eye. The nitromethane possibly forms the 



, J~ : : . 



salt CH 3 N— ONa by double decomposition. 



